Shcharbin Dzmitry, Ionov Maksim, Abashkin Viktar, Loznikova Svetlana, Dzmitruk Volha, Shcharbina Natallia, Matusevich Ludmila, Milowska Katarzyna, Gałęcki Krystian, Wysocki Stanisław, Bryszewska Maria
Institute of Biophysics and Cell Engineering of NASB, Akademicheskaja st. 27, 220072 Minsk, Belarus.
Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska st. 141/133, 90-236 Lodz, Poland.
Colloids Surf B Biointerfaces. 2015 Oct 1;134:377-83. doi: 10.1016/j.colsurfb.2015.07.017. Epub 2015 Jul 17.
Protein absorption at the surface of big nanoparticles and formation of 'protein corona' can completely change their biological properties. In contrast, we have studied the binding of small nanoparticles - dendrimers - to proteins and the formation of their 'nanoparticle corona'. Three different types of interactions were observed. (1) If proteins have rigid structure and active site buried deeply inside, the 'nanoparticle corona' is unaffected. (2) If proteins have a flexible structure and their active site is also buried deeply inside, the 'nanoparticle corona' affects protein structure, but not enzymatic activity. (3) The 'nanoparticle corona' changes both the structure and enzymatic activity of flexible proteins that have surface-based active centers. These differences are important in understanding interactions taking place at a bio-nanointerface.
大尺寸纳米颗粒表面的蛋白质吸附以及“蛋白质冠”的形成能够彻底改变其生物学特性。相比之下，我们研究了小尺寸纳米颗粒（树枝状大分子）与蛋白质的结合以及它们“纳米颗粒冠”的形成。观察到三种不同类型的相互作用。（1）如果蛋白质具有刚性结构且活性位点深埋于内部，则“纳米颗粒冠”不受影响。（2）如果蛋白质具有柔性结构且其活性位点也深埋于内部，则“纳米颗粒冠”会影响蛋白质结构，但不影响酶活性。（3）“纳米颗粒冠”会改变具有基于表面活性中心的柔性蛋白质的结构和酶活性。这些差异对于理解生物纳米界面处发生的相互作用很重要。
