Centre for Cellular and Molecular Biology (CSIR-CCMB), Hyderabad, Telangana State, India.
Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, OH, USA.
Adv Exp Med Biol. 2018;1048:175-198. doi: 10.1007/978-3-319-72041-8_11.
The physico-chemical properties of nanoparticles, as characterized under idealized laboratory conditions, have been suggested to differ significantly when studied under complex physiological environments. A major reason for this variation has been the adsorption of biomolecules (mainly proteins) on the nanoparticle surface, constituting the so-called "biomolecular corona". The formation of biomolecular corona on the nanoparticle surface has been reported to influence various nanoparticle properties viz. cellular targeting, cellular interaction, in vivo clearance, toxicity, etc. Understanding the interaction of nanoparticles with proteins upon administration in vivo thus becomes important for the development of effective nanotechnology-based platforms for biomedical applications. In this chapter, we describe the formation of protein corona on nanoparticles and the differences arising in its composition due to variations in nanoparticle properties. Also discussed is the influence of protein corona on various nanoparticle activities.
纳米粒子的物理化学性质在理想化的实验室条件下进行了表征，当在复杂的生理环境下进行研究时，这些性质被认为会有很大的不同。造成这种变化的一个主要原因是生物分子（主要是蛋白质）在纳米粒子表面的吸附，构成了所谓的“生物分子冠”。据报道，纳米粒子表面生物分子冠的形成会影响各种纳米粒子的性质，如细胞靶向、细胞相互作用、体内清除、毒性等。因此，了解纳米粒子在体内给药时与蛋白质的相互作用对于开发基于纳米技术的有效生物医学应用平台变得非常重要。在本章中，我们描述了纳米粒子上蛋白质冠的形成，以及由于纳米粒子性质的变化而导致其组成上的差异。还讨论了蛋白质冠对各种纳米粒子活性的影响。
