Chemical and Biochemical Process Engineering Unit, Faculty of Engineering, University of Mons, 7000 Mons, Belgium.
Unite Recherche et Developpement Agro-Biotechnologies Industrielles (URD ABI), Centre Europeen de Biotechnologie et Bieconomie (CEBB), AgroParisTech, 51110 Pomacle, France.
Mar Drugs. 2022 Apr 13;20(4):264. doi: 10.3390/md20040264.
Projections show that the cultivation of microalgae will extend to the production of bio-based compounds, such as biofuels, cosmetics, and medicines. This will generate co-products or residues that will need to be valorized to reduce the environmental impact and the cost of the process. This study explored the ability of lipid-extracted residue as a sole carbon and nitrogen source for growing oleaginous yeasts without any pretreatment. Both wild-type W29 and mutant JMY3501 (which was designed to accumulate more lipids without their remobilization or degradation) showed a similar growth rate of 0.28 h at different pH levels (3.5, 5.5, and 7.5). However, the W29 cell growth had the best cell number on microalgal residue at a pH of 7.5, while three times fewer cells were produced at all pH levels when JMY3501 was grown on microalgal residue. The JMY3501 growth curves were similar at pH 3.5, 5.5, and 7.5, while the fatty-acid composition differed significantly, with an accumulation of α-linolenic acid on microalgal residue at a pH of 7.5. Our results demonstrate the potential valorization of residue for growth and the positive effect of a pH of 7.5 on the fatty acid profile.
预测表明，微藻的培养将扩展到生物基化合物的生产，如生物燃料、化妆品和药品。这将产生需要增值的副产物或残渣，以减少对环境的影响和降低过程成本。本研究探讨了提取脂质后的残渣作为唯一的碳氮源，在未经任何预处理的情况下培养产油酵母的能力。野生型 W29 和突变体 JMY3501（设计为在不进行再动员或降解的情况下积累更多的脂质）在不同的 pH 值（3.5、5.5 和 7.5）下的生长速率相似，均为 0.28 h。然而，在 pH 值为 7.5 时，W29 细胞在微藻残渣上的生长具有最佳的细胞数量，而当 JMY3501 在微藻残渣上生长时，在所有 pH 值下产生的细胞数量减少了三倍。在 pH 值为 3.5、5.5 和 7.5 时，JMY3501 的生长曲线相似，而脂肪酸组成则有显著差异，在 pH 值为 7.5 时，α-亚麻酸在微藻残渣上的积累量增加。我们的结果表明，残渣具有用于生长的增值潜力，pH 值为 7.5 对脂肪酸谱有积极影响。
