Cortés-Lara Sara, Urdiain Mercedes, Mora-Ruiz Merit, Prieto Laura, Rosselló-Móra Ramon
Marine Microbiology Group, Department of Ecology and Marine Resources, Mediterranean Institute for Advanced Studies (IMEDEA, CSIC-UIB), Esporles, Spain.
Ecosystem Oceanography Group, Department of Ecology and Coastal Management, Andalusian Marine Science Institute (ICMAN-CSIC), Republica Saharaui 2, 11519 Puerto Real (Cádiz), Spain.
Syst Appl Microbiol. 2015 Oct;38(7):494-500. doi: 10.1016/j.syapm.2015.07.001. Epub 2015 Jul 20.
The microbiota associated to the gastric cavity of four exemplars of the jellyfish Cotylorhiza tuberculata has been studied by means of cultured-dependent and -independent methods. The pyrosequencing approach rendered a very reduced diversity of Bacteria with four major groups shared by the four exemplars that made up to 95% of the total diversity. The culturing approach recovered low abundant organisms and some of them also detected by the pyrosequencing approach. The major key organisms were related to the genera Spiroplasma, Thalassospira, Tenacibaculum (from the pyrosequencing data), and Vibrio (from the cultivable fraction). Altogether the results indicate that C. tuberculata harbors an associated microbiota of very reduced diversity. On the other hand, some of the major key players may be potential pathogens and the host may serve as dispersal mechanism.
利用依赖培养和不依赖培养的方法，对4个地中海球栉水母样本胃腔中的微生物群进行了研究。焦磷酸测序方法显示细菌多样性极低，4个样本共有4个主要菌群，占总多样性的95%。培养方法获得了低丰度微生物，其中一些也通过焦磷酸测序方法检测到。主要的关键微生物与螺旋体属、海旋菌属、黄杆菌属（来自焦磷酸测序数据）和弧菌属（来自可培养部分）有关。总体结果表明，地中海球栉水母拥有多样性极低的相关微生物群。另一方面，一些主要的关键参与者可能是潜在病原体，而宿主可能充当传播机制。
