Orvieto Raoul, Dratviman-Storobinsky Olga, Cohen Yoram
Department of Obstetrics and Gynecology, Chaim Sheba Medical Center (Tel Hashomer), Ramat Gan, Israel.
Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
Am J Reprod Immunol. 2015 Nov;74(5):392-7. doi: 10.1111/aji.12416. Epub 2015 Jul 28.
Ovarian hyperstimulation syndrome (OHSS) is similar to vascular leak syndrome (VLS), which may be attributable to the massive increase in systemic inflammatory cytokines. The hyperstimulated human ovaries were demonstrated to contain interleukin (IL)-2, which, in turn, was suggested to activate the systemic inflammatory response characteristic of OHSS. As the source of follicular fluid IL-2 is still unclear, in the present study, we sought to validate the presence of IL-2 and IL-2 mRNA expression in human luteinized granulosa cells.
IL-2 nuclear expression was detected using real-time PCR and immunofluorescence staining of human luteinized granulosa cell from 6 patients undergoing in vitro fertilization treatment. Calretinin immunofluorescence staining was used as a marker of granulosa cells.
IL-2-positive immunofluorescence staining was detected within nuclei of granulosa cells, together with positive stain for calretinin, confirming the presence of granulosa cell. Moreover, IL-2 gene expression was demonstrated in luteinized granulosa cells by real-time PCR.
In the present study, we provided firm evidence for the IL-2 production by human luteinized granulosa cells, as demonstrated by the presence of IL-2 and IL-2 mRNA expression in luteinized granulosa cells. Further studies are justified in an attempt to clarify the regulation and the cause-and-effect relationship between IL-2 production by the hyperstimulated ovaries and OHSS.
卵巢过度刺激综合征（OHSS）与血管渗漏综合征（VLS）相似，这可能归因于全身炎症细胞因子的大量增加。研究表明，过度刺激的人类卵巢中含有白细胞介素（IL）-2，进而有人认为它会激活OHSS特有的全身炎症反应。由于卵泡液中IL-2的来源尚不清楚，在本研究中，我们试图验证人黄素化颗粒细胞中IL-2的存在及IL-2 mRNA的表达。
采用实时PCR和免疫荧光染色法检测6例接受体外受精治疗患者的人黄素化颗粒细胞中的IL-2核表达。钙视网膜蛋白免疫荧光染色用作颗粒细胞的标志物。
在颗粒细胞核内检测到IL-2阳性免疫荧光染色，同时钙视网膜蛋白染色呈阳性，证实存在颗粒细胞。此外，通过实时PCR在黄素化颗粒细胞中证实了IL-2基因的表达。
在本研究中，我们提供了确凿证据，证明人黄素化颗粒细胞可产生IL-2，黄素化颗粒细胞中存在IL-2及IL-2 mRNA表达即证明了这一点。有必要进一步开展研究，以阐明过度刺激的卵巢产生IL-2与OHSS之间的调控及因果关系。
