松露（白松露菌）中的挥发性有机化合物：来自意大利不同地区和不同季节的样本比较
Volatile organic compounds in truffle (Tuber magnatum Pico): comparison of samples from different regions of Italy and from different seasons.
作者信息
Vita Federico, Taiti Cosimo, Pompeiano Antonio, Bazihizina Nadia, Lucarotti Valentina, Mancuso Stefano, Alpi Amedeo
机构信息
1] Department of Agriculture, Food and Environment, University of Pisa, Pisa, Italy [2] Department of Agraria, Mediterranea University of Reggio Calabria, Salita Melissari, 89124 Reggio Calabria, Italy.
LINV-Department of Plant Soil and Environmental Science, University of Florence, Florence, Italy.
出版信息
Sci Rep. 2015 Jul 30;5:12629. doi: 10.1038/srep12629.
In this paper volatile organic compounds (VOCs) from Tuber magnatum fruiting bodies were analyzed using a PTR-TOF-MS instrument. The aim was to characterize the VOC's profile of the fruiting bodies and identify if any VOCs were specific to a season and geographical areas. Multiple factorial analysis (MFA) was carried out on the signals obtained by MS. Experiments using ITS region sequencing proved that the T. magnatum life cycle includes the formation of fruiting bodies at two different times of the year. The VOCs profiles diverge when different seasonal and geographical productions are considered. Using PTR-TOF-MS, compounds present at levels as low pptv were detected. This made it possible to determine both the origin of fruiting bodies (Alba and San Miniato) and the two biological phases of fruiting bodies formation in San Miniato truffles.
本文使用质子转移反应-飞行时间质谱仪（PTR-TOF-MS）分析了白松露子实体中的挥发性有机化合物（VOCs）。目的是表征子实体的VOCs谱，并确定是否有特定于季节和地理区域的VOCs。对质谱获得的信号进行了多因素分析（MFA）。使用ITS区域测序的实验证明，白松露的生命周期包括在一年中的两个不同时间形成子实体。当考虑不同的季节和地理产量时，VOCs谱会有所不同。使用PTR-TOF-MS，可以检测到低至皮克/立方厘米水平的化合物。这使得确定子实体的来源（阿尔巴和圣米尼亚托）以及圣米尼亚托松露子实体形成的两个生物学阶段成为可能。
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