Reddyjarugu Balagangadharreddy, Pavek Todd, Southard Teresa, Barry Jason, Singh Bhupinder
Center for Animal Resources and Education, Cornell University, Ithaca, New York, USA.
Department of Biomedical Sciences, Cornell University, Ithaca, New York, USA.
J Am Assoc Lab Anim Sci. 2015 Jul;54(4):405-10.
Pain management in laboratory animals is generally accomplished by using opioids and NSAIDs. However, opioid use is hindered by controlled substance requirements and a relatively short duration of action. In this study, we compared the analgesic efficacy of firocoxib (a cyclooxygenase-2-selective NSAID) with that of buprenorphine in the mouse model of plantar incisional pain by objective measurement of mechanical allodynia and thermal hyperalgesia using von Frey and Hargreaves equipment, respectively. Our experimental design included 5 treatment groups: firocoxib at 10 mg/kg IP every 24 h (F10 group); firocoxib at 20 mg/kg IP every 24 h (F20); buprenorphine at 0.2 mg/kg SC every 8 h; intraperitoneal normal saline every 24 h; and sham group (anesthesia, no incision) treated with firocoxib at 20 mg/kg IP every 24 h (sham+F20). All mice underwent nociceptive assays at 24 h before and 4, 24, 48, and 72 h after surgery. Buprenorphine alleviated allodynia at all time points after incision. The F10 treatment alleviated allodynia at 4, 24, and 48 h, whereas F20 alleviated allodynia at 24, 48, and 72 h. None of the treatments alleviated thermal hyperalgesia at 4h. Except for F10 and buprenorphine at 24 h, all treatments alleviated thermal hyperalgesia at 24, 48, and 72 h. No significant differences were noted between the 2 doses of firocoxib and buprenorphine regarding mechanical allodynia and thermal hyperalgesia at all time points. In conclusion, the analgesic efficacy of firocoxib is comparable to that of buprenorphine in this mouse pain model.
实验动物的疼痛管理通常通过使用阿片类药物和非甾体抗炎药（NSAIDs）来实现。然而，阿片类药物的使用受到管制药物要求和相对较短作用时间的限制。在本研究中，我们通过分别使用von Frey和Hargreaves设备客观测量机械性异常性疼痛和热痛觉过敏，比较了氟罗昔康（一种环氧化酶-2选择性NSAID）与丁丙诺啡在小鼠足底切口疼痛模型中的镇痛效果。我们的实验设计包括5个治疗组：每24小时腹腔注射10 mg/kg氟罗昔康（F10组）；每24小时腹腔注射20 mg/kg氟罗昔康（F20组）；每8小时皮下注射0.2 mg/kg丁丙诺啡；每24小时腹腔注射生理盐水；以及假手术组（麻醉，无切口），每24小时腹腔注射20 mg/kg氟罗昔康（假手术+F20组）。所有小鼠在手术前24小时以及手术后4、24、48和72小时进行伤害性感受测定。丁丙诺啡在切口后的所有时间点均减轻了异常性疼痛。F10治疗在4、24和48小时减轻了异常性疼痛，而F20在24、48和72小时减轻了异常性疼痛。所有治疗在4小时均未减轻热痛觉过敏。除F10和24小时的丁丙诺啡外，所有治疗在24、48和72小时均减轻了热痛觉过敏。在所有时间点，两种剂量的氟罗昔康和丁丙诺啡在机械性异常性疼痛和热痛觉过敏方面均未观察到显著差异。总之，在该小鼠疼痛模型中，氟罗昔康的镇痛效果与丁丙诺啡相当。
