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布氏锥虫中的γ-微管蛋白复合体：分子组成、亚基相互依赖性以及轴丝中央微管蛋白装配的需求

γ-Tubulin complex in Trypanosoma brucei: molecular composition, subunit interdependence and requirement for axonemal central pair protein assembly.

作者信息

Zhou Qing, Li Ziyin

机构信息

Department of Microbiology and Molecular Genetics, University of Texas Medical School at Houston, Houston, Texas, 77030, USA.

出版信息

Mol Microbiol. 2015 Nov;98(4):667-80. doi: 10.1111/mmi.13149. Epub 2015 Sep 4.

DOI:10.1111/mmi.13149
PMID:26224545
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4636443/
Abstract

γ-Tubulin complex constitutes a key component of the microtubule-organizing center and nucleates microtubule assembly. This complex differs in complexity in different organisms: the budding yeast contains the γ-tubulin small complex (γTuSC) composed of γ-tubulin, gamma-tubulin complex protein (GCP)2 and GCP3, whereas animals contain the γ-tubulin ring complex (γTuRC) composed of γTuSC and three additional proteins, GCP4, GCP5 and GCP6. In Trypanosoma brucei, the composition of the γ-tubulin complex remains elusive, and it is not known whether it also regulates assembly of the subpellicular microtubules and the spindle microtubules. Here we report that the γ-tubulin complex in T. brucei is composed of γ-tubulin and three GCP proteins, GCP2-GCP4, and is primarily localized in the basal body throughout the cell cycle. Depletion of GCP2 and GCP3, but not GCP4, disrupted the axonemal central pair microtubules, but not the subpellicular microtubules and the spindle microtubules. Furthermore, we showed that the γTuSC is required for assembly of two central pair proteins and that γTuSC subunits are mutually required for stability. Together, these results identified an unusual γ-tubulin complex in T. brucei, uncovered an essential role of γTuSC in central pair protein assembly, and demonstrated the interdependence of individual γTuSC components for maintaining a stable complex.

摘要

γ-微管蛋白复合体是微管组织中心的关键组成部分，可引发微管组装。该复合体在不同生物体中的复杂程度有所不同：芽殖酵母含有由γ-微管蛋白、γ-微管蛋白复合体蛋白（GCP）2和GCP3组成的γ-微管蛋白小复合体（γTuSC），而动物含有由γTuSC和另外三种蛋白质GCP4、GCP5和GCP6组成的γ-微管蛋白环复合体（γTuRC）。在布氏锥虫中，γ-微管蛋白复合体的组成仍然未知，也不清楚它是否也调节表膜下微管和纺锤体微管的组装。在此我们报告，布氏锥虫中的γ-微管蛋白复合体由γ-微管蛋白和三种GCP蛋白GCP2 - GCP4组成，并且在整个细胞周期中主要定位于基体。GCP2和GCP3的缺失而非GCP4的缺失破坏了轴丝中央微管对，但未破坏表膜下微管和纺锤体微管。此外，我们表明γTuSC是两种中央对蛋白组装所必需的，并且γTuSC亚基对于稳定性相互需要。总之，这些结果确定了布氏锥虫中一种不同寻常的γ-微管蛋白复合体，揭示了γTuSC在中央对蛋白组装中的重要作用，并证明了单个γTuSC组件对于维持稳定复合体的相互依赖性。

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本文引用的文献

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The Centriole Cartwheel Protein SAS-6 in Trypanosoma brucei Is Required for Probasal Body Biogenesis and Flagellum Assembly.布氏锥虫中的中心粒车轮蛋白SAS-6是前基体生物发生和鞭毛组装所必需的。
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