Bancel Emmanuelle, Bonnot Titouan, Davanture Marlène, Branlard Gérard, Zivy Michel, Martre Pierre
INRA, UMR1095 Genetics, Diversity and Ecophysiology of Cereals, 5 chemin de Beaulieu, F-63 039 Clermont-Ferrand, France.
Blaise Pascal University, UMR1095 Genetics, Diversity and Ecophysiology of Cereals, Avenue des Landais, F-63 170 Aubière, France.
J Proteome Res. 2015 Oct 2;14(10):4432-9. doi: 10.1021/acs.jproteome.5b00446. Epub 2015 Sep 8.
The nuclear proteome of the grain of the two cultivated wheat species Triticum aestivum (hexaploid wheat; genomes A, B, and D) and T. monococcum (diploid wheat; genome A) was analyzed in two early stages of development using shotgun-based proteomics. A procedure was optimized to purify nuclei, and an improved protein sample preparation was developed to efficiently remove nonprotein substances (starch and nucleic acids). A total of 797 proteins corresponding to 528 unique proteins were identified, 36% of which were classified in functional groups related to DNA and RNA metabolism. A large number (107 proteins) of unknown functions and hypothetical proteins were also found. Some identified proteins may be multifunctional and may present multiple localizations. On the basis of the MS/MS analysis, 368 proteins were present in the two species, and in two stages of development, some qualitative differences between species and stages of development were also found. All of these data illustrate the dynamic function of the grain nucleus in the early stages of development.
利用基于鸟枪法的蛋白质组学技术，在两个发育早期阶段对两种栽培小麦——普通小麦（六倍体小麦；基因组为A、B和D）和一粒小麦（二倍体小麦；基因组为A）种子的核蛋白质组进行了分析。优化了核纯化程序，并开发了改进的蛋白质样品制备方法以有效去除非蛋白质物质（淀粉和核酸）。共鉴定出797种蛋白质，对应528种独特蛋白质，其中36%被归类到与DNA和RNA代谢相关的功能组中。还发现了大量（107种）功能未知的蛋白质和假设性蛋白质。一些鉴定出的蛋白质可能具有多种功能，并可能存在多种定位。基于串联质谱分析，在这两个物种以及两个发育阶段中均存在368种蛋白质，同时还发现了物种间和发育阶段间的一些定性差异。所有这些数据都说明了种子核在发育早期的动态功能。
