Jenkins Michael W, Watanabe Michiko, Rollins Andrew M
Department of Biomedical Engineering and Medicine, Case Western Reserve University, Cleveland, OH 44106 USA.
Department of Pediatrics, Case Western Reserve University, Cleveland, OH 44106 USA.
IEEE J Sel Top Quantum Electron. 2012 May-Jun;18(3):1166-1175. doi: 10.1109/JSTQE.2011.2166060.
Optical coherence tomography (OCT) has great potential for deciphering the role of mechanics in normal and abnormal heart development. OCT images tissue microstructure and blood flow deep into the tissue (1-2mm) at high spatiotemporal resolutions allowing unprecedented images of the developing heart. Here, we review the advancement of OCT technology to image heart development and report some of our recent findings utilizing OCT imaging under environmental control for longitudinal imaging. Precise control of the environment is absolutely required in longitudinal studies that follow the growth of the embryo or studies comparing normal versus perturbed heart development to obtain meaningful results. These types of studies are essential to tease out the influence of cardiac dynamics on molecular expression and their role in the progression of congenital heart defects.
光学相干断层扫描（OCT）在解读力学在正常和异常心脏发育中的作用方面具有巨大潜力。OCT能够以高时空分辨率对组织微观结构和深入组织（1-2毫米）的血流进行成像，从而获得发育中心脏前所未有的图像。在此，我们回顾了用于心脏发育成像的OCT技术进展，并报告了我们最近在环境控制下利用OCT成像进行纵向成像的一些发现。在跟踪胚胎生长的纵向研究或比较正常与受干扰心脏发育的研究中，绝对需要对环境进行精确控制，以获得有意义的结果。这类研究对于梳理心脏动力学对分子表达的影响及其在先天性心脏缺陷进展中的作用至关重要。
