Tucek C L, Boyd R L, Hiai H
Department of Pathology and Immunology, Monash University Medical School, Victoria, Australia.
Thymus. 1989;14(1-3):95-107.
Accumulating evidence has shown that thymic stromal-lymphoid interactions play a major role in the development of AKR thymic leukemia. A normal thymic stromal cell (TSC) line B6TE-A, which has been shown to support the in vitro growth of AKR leukemic T cells by forming multicellular complexes with them, was used to raise monoclonal antibodies. Three of these mAb, MTS 31, 32 and 38, in addition to 2 other MTS mAb, are abnormally expressed in the preleukemic and/or leukemic stages in AKR mice. These 5 MTS mAb, which detect antigens on both subpopulations of TSC and T cells, show some reduced cortical reactivity from the pre-leukemic period (MTS 32 and 35) to markedly depressed reactivity in the leukemic period (MTS 31, 32, 33, 35 and 38). While it appears that the major reduction is due to the loss of antigens from the cortical thymocytes, there is some indication that the stromal elements may be affected also. In addition, MTS 29, which was also produced in this study, while only reacting with rare thymic medullary cells in situ was densely distributed on cultured stromal cells from both normal and leukemic thymuses. In this report, the value of these MTS mAb for documenting various stages of AKR leukemogenesis has been clearly demonstrated: their possible modulatory effects on in vitro T cell leukemia growth is currently being investigated.
越来越多的证据表明，胸腺基质-淋巴细胞相互作用在AKR胸腺白血病的发展中起主要作用。一种正常的胸腺基质细胞（TSC）系B6TE-A，已被证明能通过与AKR白血病T细胞形成多细胞复合物来支持其体外生长，被用于制备单克隆抗体。其中三种单克隆抗体，MTS 31、32和38，以及另外两种MTS单克隆抗体，在AKR小鼠的白血病前期和/或白血病期异常表达。这5种MTS单克隆抗体能检测TSC和T细胞亚群上的抗原，从白血病前期（MTS 32和35）到白血病期（MTS 31、32、33、35和38），其皮质反应性有所降低，从有所降低到显著降低。虽然主要的降低似乎是由于皮质胸腺细胞抗原的丢失，但有迹象表明基质成分也可能受到影响。此外，本研究中产生的MTS 29，虽然仅与原位罕见的胸腺髓质细胞反应，但在来自正常和白血病胸腺的培养基质细胞上密集分布。在本报告中，这些MTS单克隆抗体在记录AKR白血病发生的各个阶段的价值已得到明确证明：目前正在研究它们对体外T细胞白血病生长的可能调节作用。
