Wroblewska Liliana, Kitada Tasuku, Endo Kei, Siciliano Velia, Stillo Breanna, Saito Hirohide, Weiss Ron
Department of Biological Engineering, Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
Department of Life Science Frontiers, Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan.
Nat Biotechnol. 2015 Aug;33(8):839-41. doi: 10.1038/nbt.3301. Epub 2015 Aug 3.
Synthetic regulatory circuits encoded in RNA rather than DNA could provide a means to control cell behavior while avoiding potentially harmful genomic integration in therapeutic applications. We create post-transcriptional circuits using RNA-binding proteins, which can be wired in a plug-and-play fashion to create networks of higher complexity. We show that the circuits function in mammalian cells when encoded in modified mRNA or self-replicating RNA.
由RNA而非DNA编码的合成调控回路可为控制细胞行为提供一种方法，同时在治疗应用中避免潜在有害的基因组整合。我们利用RNA结合蛋白创建转录后回路，这些回路可以以即插即用的方式连接起来，以创建更复杂的网络。我们表明，当这些回路由修饰的mRNA或自我复制RNA编码时，它们在哺乳动物细胞中发挥作用。
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