Second Department of Hepatobiliary Surgery, Zhujiang Hospital, State Key Laboratory of Organ Failure Research, Co-Innovation Center for Organ Failure Research , Southern Medical University , Guangzhou , China.
Department of Gastroenterology , Peking University Third Hospital , Beijing , China.
ACS Synth Biol. 2020 Feb 21;9(2):269-282. doi: 10.1021/acssynbio.9b00313. Epub 2020 Jan 9.
Owing to superior safety, the RNA-only delivery synthetic circuit is more suitable for cell-based medicine. Modules which possess matching inputs and outputs could be strung by daisy-chaining to compose RNA-only delivery synthetic gene circuits. In this study, we engineered well-characterized biological parts to construct composable modules, each of which could receive signals from the upstream module and transmit the processed signal to the next module using standard interfaces. Capsid-cNOT7, through which logic gates could be changed by merely changing the type of it, was used as the core element for logical process. Daisy chain topology was used to build RNA-only delivery mammalian synthetic circuits which possess validated functions such as fan out, protein sensing, drug sensing, light sensing, 2-input logic gate, 3-input logic gate, and volatile memory, providing a new method to simplify the design of RNA-only delivery synthetic circuits.
由于具有优越的安全性，仅 RNA 递送的合成电路更适合细胞医学。具有匹配输入和输出的模块可以通过级联连接成仅 RNA 递送的合成基因电路。在这项研究中，我们设计了经过充分表征的生物部件来构建可组合模块，每个模块都可以从前一个模块接收信号，并使用标准接口将处理后的信号传输到下一个模块。衣壳-cNOT7 可通过改变其类型来改变逻辑门，被用作逻辑处理的核心元件。级联拓扑结构被用于构建具有已验证功能的仅 RNA 递送哺乳动物合成电路，例如扇出、蛋白质感应、药物感应、光感应、2 输入逻辑门、3 输入逻辑门和易失性存储器，为简化仅 RNA 递送合成电路的设计提供了一种新方法。
