犬腺病毒1型和2型早期区域1的鉴定及核苷酸序列

Identification and nucleotide sequence of the early region 1 from canine adenovirus types 1 and 2.

作者信息

Spibey N, McClory R S, Cavanagh H M

机构信息

Department of Veterinary Pathology, University of Glasgow Veterinary School, Scotland, U.K.

出版信息

Virus Res. 1989 Nov;14(3):241-55. doi: 10.1016/0168-1702(89)90005-1.

DOI:10.1016/0168-1702(89)90005-1

PMID:2623943

Abstract

The genome of canine adenovirus type 1 (CAV-1) has been cloned and restriction maps compiled. These maps are compared with those of canine adenovirus type 2 (CAV-2). The left ends of both genomes were further characterised by DNA sequence analysis. Several features of the DNA sequence and predicted polypeptide sequence are similar to those of the human adenoviruses. The level of homology observed across the E1 regions appears to be of the same order as the overall DNA similarity between CAV-1 and CAV-2 (75%). Transfection experiments using the presumptive E1a containing region of CAV-2 suggests that it encodes a transactivating function typical of the human adenovirus E1a genes.

摘要

犬腺病毒1型（CAV-1）的基因组已被克隆并绘制了限制性图谱。这些图谱与犬腺病毒2型（CAV-2）的图谱进行了比较。通过DNA序列分析进一步对两个基因组的左端进行了特征描述。DNA序列和预测的多肽序列的几个特征与人腺病毒的相似。在E1区域观察到的同源性水平似乎与CAV-1和CAV-2之间的总体DNA相似性（75%）处于同一水平。使用CAV-2推定的含E1a区域进行的转染实验表明，它编码一种典型的人腺病毒E1a基因的反式激活功能。