犬腺病毒2型E1区的核苷酸序列。

Nucleotide sequence of E1 region of canine adenovirus type 2.

作者信息

Shibata R, Shinagawa M, Iida Y, Tsukiyama T

机构信息

Department of Veterinary Public Health, School of Veterinary Medicine, Obihiro University of Agriculture, Hokkaido, Japan.

出版信息

Virology. 1989 Oct;172(2):460-7. doi: 10.1016/0042-6822(89)90188-8.

DOI:10.1016/0042-6822(89)90188-8

PMID:2800332

Abstract

The nucleotide sequence of the leftmost EcoRI-C fragment (0 to 11.3%) of canine adenovirus type 2 (CAd2) which could transform rodent cells morphologically but required additional sequences from 10 to 32 map units (m.u.) for full expression of its oncogenic potential was determined. The EcoRI-C fragment contains 3609 nucleotide base pairs (bp) encoding E1A, E1B, and pIX genes. Although the nucleotide sequence of CAd2 E1 shows little homology to those of human Ads, the amino acid sequences of the E1 proteins predicted from nucleotide sequence of CAd2 E1 and those for human and simian Ads are partially conserved.

摘要

测定了犬腺病毒2型（CAd2）最左侧EcoRI-C片段（0至11.3%）的核苷酸序列，该片段可在形态上转化啮齿动物细胞，但需要10至32个图距单位（m.u.）的额外序列才能充分表达其致癌潜能。EcoRI-C片段包含3609个核苷酸碱基对（bp），编码E1A、E1B和pIX基因。虽然CAd2 E1的核苷酸序列与人类腺病毒的核苷酸序列几乎没有同源性，但根据CAd2 E1的核苷酸序列预测的E1蛋白的氨基酸序列与人类和猴腺病毒的E1蛋白氨基酸序列部分保守。