苜蓿银纹夜蛾多粒包埋核多角体病毒感染期间草地贪夜蛾细胞的比较蛋白质组学分析
Comparative proteomics analysis of Spodoptera frugiperda cells during Autographa californica multiple nucleopolyhedrovirus infection.
作者信息
Yu Qian, Xiong Youhua, Gao Hang, Liu Jianliang, Chen Zhiqiang, Wang Qin, Wen Dongling
机构信息
College of Food Science and Technology, Zhongkai University of Agriculture and Engineering, No. 501 Zhongkai Road, Haizhu District, Guangdong, 510225, People's Republic of China.
出版信息
Virol J. 2015 Aug 4;12:115. doi: 10.1186/s12985-015-0346-9.
BACKGROUND
Increasing evidence sugggest that in addition of balculovirus controling insect host, host cells also responds to balculovirus infection. However, compared to existing knowledge on virus gene, host cell responses are relatively poorly understood.
METHODS
In this study, Spodoptera frugiperda (Sf9) cells were infected with Autographa californica multiple nucleopolyhedrovirus (AcMNPV). The protein composition and protein changes of Spodoptera frugiperda (Sf9) cells of different infection stages were analysed by isobaric tag for relative and absolute quantification (iTRAQ) techniques.
RESULTS
A total of 4004 Sf9 proteins were identified by iTRAQ and 413 proteins were found as more than 1.5-fold changes in abundance. The 413 proteins were categorised according to GO classification for insects and were categorised into: biological process, molecular function and cellular component.
CONCLUSIONS
The determination of the protein changes in infected Sf9 cells would help to better understanding of host cell responses and facilitate better design of this virus-host cell interaction in pest insect control and other related fields.
背景
越来越多的证据表明，除了杆状病毒控制昆虫宿主外，宿主细胞也会对杆状病毒感染作出反应。然而，与现有的病毒基因知识相比，宿主细胞的反应相对了解较少。
方法
在本研究中，用苜蓿银纹夜蛾多核多角体病毒（AcMNPV）感染草地贪夜蛾（Sf9）细胞。采用相对和绝对定量等压标签（iTRAQ）技术分析不同感染阶段草地贪夜蛾（Sf9）细胞的蛋白质组成和蛋白质变化。
结果
通过iTRAQ共鉴定出4004种Sf9蛋白，发现413种蛋白的丰度变化超过1.5倍。根据昆虫的基因本体分类（GO）对这413种蛋白进行分类，分为：生物学过程、分子功能和细胞成分。
结论
确定感染的Sf9细胞中的蛋白质变化将有助于更好地理解宿主细胞反应，并有助于在害虫防治和其他相关领域更好地设计这种病毒-宿主细胞相互作用。
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