Yu Qian, Xiong Youhua, Liu Jianliang, Wang Qin, Qiu Yuanxin, Wen Dongling
College of Food Science and Technology, Zhongkai University of Agriculture and Engineering, Guangdong, 510225, PR China.
College of Food Science and Technology, Zhongkai University of Agriculture and Engineering, Guangdong, 510225, PR China.
Comp Biochem Physiol Part D Genomics Proteomics. 2016 Jun;18:21-9. doi: 10.1016/j.cbd.2016.01.008. Epub 2016 Feb 3.
Infection with Autographa californica multiple nucleopolyhedrovirus (AcMNPV) mutants lacking a functional p35 gene can induce host cell apoptosis, which provides the possibility to use the potential of these viruses in the biological control of pest insects. Nonetheless, the proteomics or the protein changes of Spodoptera frugiperda (Sf9) cells infected with p35 knockout AcMNPV have not yet been studied. To further improve the use of AcMNPV, we set out to analyze the protein composition and protein changes of Sf9 cells of different infection stages by isobaric tag for relative and absolute quantification (iTRAQ) techniques. A total of 4004 sf9 proteins were identified by iTRAQ. After comparation of the significantly expressed 483 proteins from p35koAcMNPV-infected Sf9 cells and the significantly expressed 413 proteins from wtAcMNPV-infected Sf9 cells, we found that 226 proteins were specific to p35koAcMNPV-infected Sf9 cells. The 226 proteins were categorized according to GO classification for insects and were categorized into: biological processes, molecular functions and cellular components. Of interest, the most up-regulated proteins related to Epstein-Barr virus infection, RNA transport, Calcium signaling pathway, cGMP-PKG signaling pathway, oxidative phosphorylation and N-Glycan biosynthesis. Determination of the protein changes in p35 knockout AcMNPV-infected Sf9 cells would facilitate the better use of this virus-host cell interaction in pest insect control and other related fields.
感染缺乏功能性p35基因的苜蓿银纹夜蛾多核多角体病毒（AcMNPV）突变体可诱导宿主细胞凋亡，这为利用这些病毒在害虫生物防治中的潜力提供了可能。尽管如此，尚未对感染p35基因敲除型AcMNPV的草地贪夜蛾（Sf9）细胞的蛋白质组学或蛋白质变化进行研究。为了进一步提高AcMNPV的利用效率，我们着手通过相对和绝对定量等压标签（iTRAQ）技术分析不同感染阶段Sf9细胞的蛋白质组成和蛋白质变化。通过iTRAQ共鉴定出4004种Sf9蛋白。在比较p35基因敲除型AcMNPV感染的Sf9细胞中显著表达的483种蛋白和野生型AcMNPV感染的Sf9细胞中显著表达的413种蛋白后，我们发现226种蛋白是p35基因敲除型AcMNPV感染的Sf9细胞所特有的。这226种蛋白根据昆虫的基因本体（GO）分类进行归类，分为生物过程、分子功能和细胞成分。有趣的是，上调最多的蛋白与爱泼斯坦-巴尔病毒感染、RNA转运、钙信号通路、cGMP-PKG信号通路、氧化磷酸化和N-聚糖生物合成有关。确定p35基因敲除型AcMNPV感染的Sf9细胞中的蛋白质变化将有助于在害虫防治和其他相关领域更好地利用这种病毒-宿主细胞相互作用。
