偶氮胂III在生理条件下与钙形成2:1络合物，与镁形成1:1络合物。表观解离常数的估计值。
Arsenazo III forms 2:1 complexes with Ca and 1:1 complexes with Mg under physiological conditions. Estimates of the apparent dissociation constants.
作者信息
Thomas M V
出版信息
Biophys J. 1979 Mar;25(3):541-8. doi: 10.1016/S0006-3495(79)85322-9.
Abstract
Experiments to determine the apparent dissociation constants of the Ca and Mg complexes of arsenazo III clearly indicated that the predominant Ca complex contains one Ca ion and two dye molecules, although previous reports have either claimed or assumed 1:1 complexing. The evidence is based on the effects of varying [dye] as well as [Ca] and [Mg], and clear evidence for the formation of 1:1 complexes with Ca was obtained only at submicromolar [dye], whereas Mg formed 1:1 complexes exclusively. The implications of these findings with regard to the use of arsenazo III as an indicator of intracellular free [Ca] are discussed, with particular reference to its selectivity for Ca and the interference effects of other ions.
摘要
测定偶氮胂III钙和镁配合物的表观解离常数的实验清楚地表明，主要的钙配合物包含一个钙离子和两个染料分子，尽管先前的报告要么声称要么假定为1:1络合。证据基于改变[染料]以及[钙]和[镁]的影响，只有在亚微摩尔[染料]浓度下才获得了与钙形成1:1配合物的明确证据，而镁则只形成1:1配合物。讨论了这些发现对于使用偶氮胂III作为细胞内游离[钙]指示剂的意义，特别提到了其对钙的选择性以及其他离子的干扰效应。
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