金属变色指示剂染料偶氮胂III与咖啡因形成1:1配合物。
The metallochromic indicator dye Arsenazo III forms 1:1 complexes with caffeine.
作者信息
Best P M, Abramcheck C W
出版信息
Biochim Biophys Acta. 1985 Jan 28;838(1):179-82. doi: 10.1016/0304-4165(85)90265-x.
PMID:3967045
Abstract
The metallochromic indicator dye, Arsenazo III, forms a 1:1 complex with caffeine, a methylxanthine. Binding is accompanied by a wavelength-dependent shift in the absorption spectrum of the dye. The magnitude of the absorption change is significant at wavelengths typically used to monitor intracellular calcium ion. The equilibrium constant for the caffeine-dye reaction is approx. 20 mM. The complex has a differential molar extinction coefficient of -5.05 X 10(3) M-1 X cm-1 at 630 nm.
摘要
金属显色指示剂染料偶氮胂III与甲基黄嘌呤咖啡因形成1:1的复合物。结合过程中染料吸收光谱会发生与波长相关的偏移。在通常用于监测细胞内钙离子的波长处，吸收变化幅度显著。咖啡因与染料反应的平衡常数约为20 mM。该复合物在630 nm处的微分摩尔消光系数为-5.05×10³ M⁻¹·cm⁻¹。
Zotero 插件