脂质双分子层中的能量转移。
Energy transfer in lipid bilayers.
作者信息
Estep T N, Thompson T E
出版信息
Biophys J. 1979 May;26(2):195-207. doi: 10.1016/S0006-3495(79)85244-3.
Abstract
The quenching of fluorescence due to energy transfer between a dilute, random array of donor and acceptor chromophores in lipid bilayer was measured and compared to theoretical expressions developed to predict the decrease in emission intensity under these circumstances. The observed intensity was found to be the same function of quencher concentration in both planar, multilamellar dispersions and small, spherical vesicles. The degree of quenching was accurately predicted by a simple relation derived in this paper, as well as a more complex equation previously developed by Tweet, et al. The results suggest that significant quenching may be observed even when the average donor-acceptor separation exceeds the Förster critical distance by severalfold. Application of these results to problems of current interest in membrane research are discussed.
摘要
测量了脂质双层中供体和受体发色团的稀疏散乱阵列之间能量转移导致的荧光猝灭，并将其与为预测这种情况下发射强度降低而推导的理论表达式进行了比较。在平面多层层状分散体和小的球形囊泡中，观察到的强度是猝灭剂浓度的相同函数。本文推导的一个简单关系式以及Tweet等人先前推导的一个更复杂的方程式，都能准确预测猝灭程度。结果表明，即使平均供体 - 受体间距比Förster临界距离大几倍，也可能观察到显著的猝灭。讨论了这些结果在当前膜研究热点问题中的应用。
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