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表面荧光共振能量转移的计算

Calculation on fluorescence resonance energy transfer on surfaces.

作者信息

Dewey T G, Hammes G G

出版信息

Biophys J. 1980 Dec;32(3):1023-35. doi: 10.1016/S0006-3495(80)85033-8.

Abstract

A general method for estimating fluorescence resonance energy transfer between distributions of donors and acceptors on surfaces is presented. Continued fraction approximants are obtained from equivalent power series expansions of the change in quantum yield in terms of the fluorescent lifetimes or the steady-state fluorescence. These approximants provide analytic equations for the analysis of energy transfer and error bounds for the approximants. Specific approximants are derived for five models of interest for membrane biochemistry: (a) an infinite plane, (b) parallel infinite planes, (c) the surface of a sphere, (d) the surfaces of concentric spheres, and (e) the surfaces of two separated spheres. Recent experimental results in the literature are analyzed with the equations obtained.

摘要

提出了一种估算表面供体和受体分布之间荧光共振能量转移的通用方法。通过荧光寿命或稳态荧光量子产率变化的等效幂级数展开式得到连分数近似值。这些近似值为能量转移分析提供了解析方程以及近似值的误差界限。针对膜生物化学感兴趣的五种模型推导出了特定的近似值:(a)无限平面,(b)平行无限平面,(c)球体表面,(d)同心球体表面,以及(e)两个分离球体表面。用所得方程对文献中最近的实验结果进行了分析。

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