Hoffmann A, Metzger J, Wöhlke A, Peters M, Junginger J, Mischke R, Distl O, Hewicker-Trautwein M
Department of Pathology, University of Veterinary Medicine Hannover, Hannover, Germany.
Institute for Animal Breeding and Genetics, University of Veterinary Medicine Hannover, Germany.
Vet Pathol. 2016 May;53(3):614-20. doi: 10.1177/0300985815595516. Epub 2015 Aug 4.
The present study describes a generalized congenital skin condition in 14 Great Dane puppies. Macroscopically, all dogs showed generalized gray to yellow scaling and skin wrinkles on the head and all 4 extremities. Skin sections were histologically examined using hematoxylin and eosin, Heidenhain's Azan, and Sudan red III staining methods and by conducting the alcian blue/periodic acid Schiff (AB/PAS) reaction technique on sections. Furthermore, incubation with hyaluronidase was performed. Skin samples were ultrastructurally analyzed using transmission electron microscopy. All affected Great Dane puppies had epidermal and follicular orthokeratotic hyperkeratosis, enlarged keratohyaline granules, vacuolated keratinocytes, and accumulations of an eosinophilic and alcianophilic, lipid-rich material within dilated hair follicular lumina and the cytoplasm of sebocytes. The macroscopic, histopathologic, and ultrastructural skin changes in all 14 Great Dane puppies indicate a new variant of a primary disorder of cornification with congenital, non-epidermolytic, lamellar ichthyosiform appearance.
本研究描述了14只大丹犬幼犬的一种全身性先天性皮肤疾病。宏观上，所有犬只头部和四肢均出现全身性灰色至黄色鳞屑及皮肤褶皱。采用苏木精和伊红染色、海登海因氏偶氮蓝染色、苏丹红III染色方法以及对切片进行阿尔辛蓝/过碘酸希夫（AB/PAS）反应技术对皮肤切片进行组织学检查。此外，还进行了透明质酸酶孵育。使用透射电子显微镜对皮肤样本进行超微结构分析。所有患病大丹犬幼犬均有表皮和毛囊正角化性角化过度、透明角质颗粒增大、角质形成细胞空泡化以及在扩张的毛囊腔和皮脂腺细胞胞质内有嗜酸性和嗜阿尔辛蓝的富含脂质物质积聚。所有14只大丹犬幼犬的宏观、组织病理学和超微结构皮肤变化表明这是一种先天性、非表皮松解性、板层状鱼鳞病样外观的原发性角化障碍的新变体。
