Alger J R, Prestegard J H
Biophys J. 1979 Oct;28(1):1-13. doi: 10.1016/S0006-3495(79)85154-1.
The permeation of acetic acid through large unilamellar phospholipid vesicle membranes has been investigated using the unique capability of nuclear magnetic resonance to characterize flow under pseudo-equilibrium conditions. Two types of experiments have been employed: total line shape analysis and selective population transfer. These techniques are sensitive to permeation on time scales ranging form 0.001 to 10.0 s. The permeation rate dependence on pH and acetic acid concentration indicates that the neutral acetic acid monomer is the dominant permeant species with a permeation coefficient of 5 +/- 2 x 10-4 cm/s. Mechanisms of permeation and the applicability of nuclear magnetic resonance methodology are discussed.
利用核磁共振在准平衡条件下表征流动的独特能力，研究了乙酸通过大单层磷脂囊泡膜的渗透情况。采用了两种类型的实验：全线形分析和选择性布居转移。这些技术对0.001至10.0秒时间尺度上的渗透敏感。渗透速率对pH值和乙酸浓度的依赖性表明，中性乙酸单体是主要的渗透物种，渗透系数为5±2×10⁻⁴厘米/秒。讨论了渗透机制和核磁共振方法的适用性。
