磷脂囊泡中马来酸渗透系数的核磁共振测定
Nuclear magnetic resonance determinations of permeation coefficients for maleic acid in phospholipid vesicles.
作者信息
Prestegard J H, Cramer J A, Viscio D B
出版信息
Biophys J. 1979 Jun;26(3):575-84. doi: 10.1016/s0006-3495(79)85272-8.
Abstract
Lipid bilayer permeation coefficients for the neutral maleic acid molecule and the maleate monoanion have been determined by proton magnetic resonance techniques. Phosphatiydylcholine-cholesterol (2:1) unilamellar vesicles were prepared having an initial maleate anion concentration gradient stabilized by coupling to an impermeant potassium counterion. The coupling was released by addition of valinomycin, and the time evolution of external pH, internal pH, and maleate concentration followed using nuclear magnetic resonance areas and chemical shifts. Transport rate equations were numerically integrated to fit the date, yielding best fit permeation coefficients of 4 X 10(-9) and 4 X 10(-5) cm/s for maleate monoanion and maleic acid, respectively.
摘要
已通过质子磁共振技术测定了中性马来酸分子和马来酸单阴离子的脂质双层渗透系数。制备了磷脂酰胆碱 - 胆固醇（2:1）单层囊泡，其初始马来酸根阴离子浓度梯度通过与非渗透性钾抗衡离子偶联而稳定。通过添加缬氨霉素释放偶联，利用核磁共振面积和化学位移跟踪外部pH、内部pH和马来酸根浓度的时间变化。对传输速率方程进行数值积分以拟合数据，得出马来酸单阴离子和马来酸的最佳拟合渗透系数分别为4×10⁻⁹和4×10⁻⁵cm/s。
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