Institute of Farm Product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; College of Forestry, Northeast Forestry University, Harbin 150040, China.
Ultrason Sonochem. 2014 Jan;21(1):98-103. doi: 10.1016/j.ultsonch.2013.06.004. Epub 2013 Jun 15.
The ultrasound-assisted synthesis of lutein disuccinate from all-trans lutein (AL) and succinic anhydride (SA) was investigated in this study. Triethylamine was used as the catalyst. Based on the single-factor experiments, a 7-level-3-factor uniform design and response surface analysis were further employed to evaluate the effects of the selected variables including molar ratio of SA/AL, reaction time and ultrasonic power on the yield of lutein disuccinate. The results indicated that the data were adequately fitted into a second-order polynomial model; the molar ratio of SA/AL significantly affected the synthesis of lutein disuccinate, whereas reaction time and ultrasonic power did not. Based on ridge max analysis, the optimum condition for lutein disuccinate synthesis was predicted to be the molar ratio of SA/AL 265.3:1, ultrasonic power 300 W and reaction time 131.6 min with the lutein disuccinate yield of 80.53±0.18%, which give a 43.8% increase compared with the traditional method, and also significantly shorten the reaction time.
本研究采用超声辅助法，以全反式叶黄素（AL）和琥珀酸酐（SA）为原料合成叶黄素双琥珀酸酯。三乙胺作为催化剂。在单因素实验的基础上，进一步采用 7 水平 3 因素均匀设计和响应面分析，考察了琥珀酸酐/叶黄素摩尔比、反应时间和超声功率等因素对叶黄素双琥珀酸酯产率的影响。结果表明，数据能够很好地拟合为二次多项式模型；琥珀酸酐/叶黄素摩尔比对叶黄素双琥珀酸酯的合成有显著影响，而反应时间和超声功率的影响不显著。基于脊最大值分析，预测叶黄素双琥珀酸酯合成的最佳条件为琥珀酸酐/叶黄素摩尔比 265.3:1、超声功率 300 W、反应时间 131.6 min，在此条件下叶黄素双琥珀酸酯的产率为 80.53±0.18%，与传统方法相比，产率提高了 43.8%，同时反应时间也显著缩短。
