采用高效液相色谱法同时测定韩方当归四物汤中9种标志物成分。

Simultaneous determination of nine marker compounds in the traditional Korean medicine, Dangguisu-san by high-performance liquid chromatography.

作者信息

Seo Chang-Seob, Shin Hyeun-Kyoo

机构信息

Herbal Medicine Formulation Research Group, Herbal Medicine Research Division, Korea Institute, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon, 305-811, Republic of Korea.

出版信息

Pharmacogn Mag. 2015 Jul-Sep;11(43):555-61. doi: 10.4103/0973-1296.160457.

DOI:10.4103/0973-1296.160457

PMID:26246731

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4522842/

Abstract

BACKGROUND

Dangguisu-san (DGSS) has been widely used to treat ecchymosis, blood stagnation and pain resulting from physical shock in Korea.

OBJECTIVE

A high-performance liquid chromatography-photodiode array detection (HPLC-PDA) method for simultaneous analysis of nine components, albiflorin (1), paeoniflorin (2), liquiritin (3), nodakenin (4), coumarin (5), liquiritigenin (6), cinnamic acid (7), cinnamaldehyde (8), and glycyrrhizin (9) in DGSS extract has been developed for the first time.

MATERIALS AND METHODS

The analytical column for separation of the nine constituents used a Gemini C18 column kept at 40°C by the gradient elution with 1.0% (v/v) acetic acid in water and 1.0% (v/v) acetic acid in acetonitrile as mobile phase. The flow rate was 1.0 mL/min and the injection volume was 10 μL.

RESULTS

Calibration curves of all compounds showed good linearity (r (2) ≥ 0.9999) within the test ranges. The limits of detection and quantification for all analytes were 0.01-0.27 μg/mL and 0.04-0.89 μg/mL, respectively. All recoveries of the nine marker compounds were 96.62-102.47% with relative standard deviations (RSD) <1.72%. The RSDs of intra-day and inter-day precision were <1.32% and 1.61%, respectively. The amounts of the nine marker components ranged from 0.10 mg/g to 13.71 mg/g.

CONCLUSION

The developed and validated HPLC-PDA method may help for the quality control of DGSS.

摘要

背景

在韩国，当归四物汤（DGSS）已被广泛用于治疗因身体冲击导致的瘀斑、血瘀和疼痛。

目的

首次建立了一种高效液相色谱 - 光电二极管阵列检测（HPLC - PDA）方法，用于同时分析DGSS提取物中的九种成分，即白花芍药苷（1）、芍药苷（2）、甘草苷（3）、紫花前胡苷（4）、香豆素（5）、甘草素（6）、肉桂酸（7）、肉桂醛（8）和甘草酸（9）。

材料与方法

用于分离这九种成分的分析柱采用Gemini C18柱，以1.0%（v/v）乙酸水溶液和1.0%（v/v）乙酸乙腈溶液作为流动相，通过梯度洗脱在40°C下进行分离。流速为1.0 mL/min，进样量为10 μL。

结果

所有化合物的校准曲线在测试范围内均显示出良好的线性（r (2)≥0.9999）。所有分析物的检测限和定量限分别为0.01 - 0.27 μg/mL和0.04 - 0.89 μg/mL。九种标记化合物的所有回收率为96.62% - 102.47%，相对标准偏差（RSD）<1.72%。日内和日间精密度的RSD分别<1.32%和1.61%。九种标记成分的含量范围为0.10 mg/g至13.71 mg/g。