Seo Chang-Seob, Kim Jung Hoon, Shin Hyeun-Kyoo
Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon, 305-811, Korea.
Afr J Tradit Complement Altern Med. 2012 Oct 1;10(1):113-23. eCollection 2012.
A simple and accurate high-performance liquid chromatographic method was applied to the quantitative analysis of seven components of the traditional herbal prescription Jaeumganghwa-tang (JGT), including 5-hydroxymethyl-2-furaldehyde, albiflorin, paeoniflorin, liquiritin, ferulic acid, nodakenin, and glycyrrhizin. All seven compounds were separated in less than 40 min on a Gemini C18 column at 40°C by gradient elution using 1.0% (v/v) aqueous acetic acid and acetonitrile containing 1.0% (v/v) acetic acid as mobile phase. The flow rate was 1.0 mL/min and the detector was a photodiode array (PDA) set at 230 nm, 254 nm, 280 nm, and 330 nm. The calibration curves showed good linearity (r (2) > 0.9998) in different concentration ranges. The recovery of each component was in the range of 91.47-102.62%, with relative standard deviations (RSDs, %) less than 4.5%. The RSDs (%) for intra- and interday precision were 0.06-2.85% and 0.06-2.83%, respectively. The concentrations of the seven components in JGT were in the range 0.74-5.48 mg/g.
一种简单准确的高效液相色谱法被应用于传统草药方剂加味姜黄汤（JGT）七种成分的定量分析，这七种成分包括5-羟甲基-2-糠醛、白花芍药苷、芍药苷、甘草苷、阿魏酸、紫花前胡苷和甘草酸。使用含1.0%（v/v）乙酸的1.0%（v/v）乙酸水溶液和乙腈作为流动相，在40℃下通过梯度洗脱，所有七种化合物在Gemini C18柱上于40分钟内分离。流速为1.0 mL/min，检测器为光电二极管阵列（PDA），设定波长为230 nm、254 nm、280 nm和330 nm。校准曲线在不同浓度范围内显示出良好的线性关系（r (2) > 0.9998）。各成分的回收率在91.47 - 102.62%范围内，相对标准偏差（RSDs，%）小于4.5%。日内和日间精密度的RSDs（%）分别为0.06 - 2.85%和0.06 - 2.83%。JGT中七种成分的浓度范围为0.74 - 5.48 mg/g。
