Seo Chang-Seob, Kim Jung Hoon, Shin Hyeun-Kyoo
Herbal Medicine Formulation Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon, Republic of Korea.
Pak J Pharm Sci. 2014 Jul;27(4):819-24.
A simple, convenient, rapid and accurate high-performance liquid chromatographic (HPLC) method was established for the simultaneous determination about three ingredients of a traditional herbal prescription, Guibi-tang (GBT): liquiritin (1), nodakenin (2) and glycyrrhizin (3). Chromatographic analysis on the three components was separated within 35 min on a Gemini C18 column and maintained at 40°C. The mobile phase consisted of water with 1.0% (v/v) acetic acid (solvent A) and acetonitrile with 1.0% (v/v) acetic acid (solvent B) in gradient mode at a flow-rate of 1.0 mL/min. Chromatograms were acquired at 254, 280 and 330 nm in a photodiode array (PDA) detector. The calibration curves showed excellent linearity (R(2)=1.0000). The average recovery of three compounds was >93.5%, with a relative standard deviation (RSD) of <2.0%. The intra-day and inter-day precision (RSD) of the three components were 0.04%-3.04% and 0.11%-2.48%, respectively. The contents of compounds 1-3 in GBT were 1.09-1.10, 2.35-2.37 and 0.81-0.82 mg/g, respectively.
建立了一种简单、方便、快速且准确的高效液相色谱（HPLC）方法，用于同时测定传统中药方剂归脾汤（GBT）中的三种成分：甘草苷（1）、紫花前胡苷（2）和甘草酸（3）。在Gemini C18柱上于40°C下35分钟内分离了这三种成分的色谱分析。流动相由含1.0%（v/v）乙酸的水（溶剂A）和含1.0%（v/v）乙酸的乙腈（溶剂B）组成，以梯度模式，流速为1.0 mL/min。在光电二极管阵列（PDA）检测器中于254、280和330 nm处采集色谱图。校准曲线显示出良好的线性（R(2)=1.0000）。三种化合物的平均回收率>93.5%，相对标准偏差（RSD）<2.0%。三种成分的日内和日间精密度（RSD）分别为0.04%-3.04%和0.11%-2.48%。GBT中化合物1-3的含量分别为1.09-1.10、2.35-2.37和0.81-0.82 mg/g。
