Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan.
Micron. 2012 Dec;43(12):1246-51. doi: 10.1016/j.micron.2012.03.011. Epub 2012 Mar 24.
This paper describes the results of the analysis of cortical rigidity in two round cell states: mitotic round cells and detached round cells after trypsinization using atomic force microscopy (AFM). These two states are primary cell events with dynamic morphological alterations in vitro. The trypsinized detached cells were fixed on the substrate of membrane anchoring oleyl surface. Fluorescent images taken by confocal laser scanning microscopy revealed diverse cell surface protrusions and cortical actin development in the round cells under different conditions. Although the cortical actin of these cells seemed to develop similarly, cortical rigidity of the trypsinized round cells showed greater stiffness than that of mitotic round cells. The elasticity measurements by AFM may detect invisible information about the maturation or strength of F-actin structures and such measurements may indicate that the strength of the actomyosin cortex would be higher in trypsinized round cells compared to mitotic cells. The mechanical properties can help provide better insights into the characteristics of the actin cytoskeleton network in vicinity of cell surface during dynamic morphological alterations.
本文通过原子力显微镜（AFM）分析了有丝分裂圆形细胞和胰蛋白酶消化后脱落的圆形细胞这两种圆形细胞状态的皮质刚性。这两种状态是体外具有动态形态变化的原代细胞事件。胰蛋白酶消化后的脱落细胞固定在膜锚定油酰表面的基质上。共聚焦激光扫描显微镜拍摄的荧光图像显示，在不同条件下，圆形细胞的细胞表面突起和皮质肌动蛋白发育呈现多样化。尽管这些细胞的皮质肌动蛋白似乎发育相似，但胰蛋白酶消化的圆形细胞的皮质刚性比有丝分裂的圆形细胞更大。AFM 的弹性测量可能会检测到关于 F-肌动蛋白结构成熟或强度的不可见信息，并且此类测量可能表明，与有丝分裂细胞相比，胰蛋白酶消化的圆形细胞中的肌动球蛋白皮质的强度更高。机械性能可以帮助更好地了解细胞表面附近肌动蛋白细胞骨架网络的特性在动态形态变化过程中。
