Gómez-Guzmán Manuel, Toral Marta, Romero Miguel, Jiménez Rosario, Galindo Pilar, Sánchez Manuel, Zarzuelo María José, Olivares Mónica, Gálvez Julio, Duarte Juan
Departamento de Farmacología, Facultad de Farmacia, Universidad de Granada, Granada, Spain.
Instituto de Investigación Biosanitaria de Granada (ibs. GRANADA), Granada, Spain.
Mol Nutr Food Res. 2015 Nov;59(11):2326-36. doi: 10.1002/mnfr.201500290. Epub 2015 Sep 2.
The cardiovascular effects of probiotics Lactobacillus fermentum CECT5716 (LC40), or L. coryniformis CECT5711 (K8) plus L. gasseri CECT5714 (LC9) (1:1) in spontaneously hypertensive rats (SHR) were evaluated.
Ten Wistar Kyoto rats (WKY) and 30 SHR were randomly assigned to four groups (n = 10): a control WKY group, a control SHR groups, an SHR group treated with LC40, and an SHR treated with K8/LC9 group for 5 weeks (at a dose of 3.3 × 10(10) colony-forming units/day in drinking water). Long-term administration of probiotics reduced systolic blood pressure. The consumption of K8/LC9 mixture significantly reduced the cardiac and renal hypertrophy. Both groups of probiotics reversed the impaired aortic endothelium-dependent relaxation to acetylcholine observed in SHR. They also abolished the increased aortic superoxide levels by reducing the increased toll-like receptor-4 mRNA levels and NADPH oxidase activity found in SHR. K8/LC9 consumption also increased endothelial nitric oxide synthase phosphorylation. Probiotic treatments induced a change in the cecum microbiota of SHR, with higher counts of the Lactobacillus spp. cluster, and lower counts of Bacteriodes spp. and Clostridium spp.
Probiotics exert cardiovascular protective effects in genetic hypertension related to the improvement of vascular pro-oxidative and pro-inflammatory status.
评估了益生菌发酵乳杆菌CECT5716（LC40）或棒状乳杆菌CECT5711（K8）加格氏乳杆菌CECT5714（LC9）（1:1）对自发性高血压大鼠（SHR）的心血管作用。
将10只Wistar Kyoto大鼠（WKY）和30只SHR随机分为四组（每组n = 10）：WKY对照组、SHR对照组、接受LC40治疗的SHR组和接受K8/LC9治疗的SHR组，持续5周（饮用水中剂量为3.3×10¹⁰菌落形成单位/天）。长期给予益生菌可降低收缩压。食用K8/LC9混合物可显著减轻心脏和肾脏肥大。两组益生菌均逆转了SHR中观察到的主动脉对乙酰胆碱的内皮依赖性舒张功能受损。它们还通过降低SHR中升高的Toll样受体4 mRNA水平和NADPH氧化酶活性，消除了主动脉超氧化物水平的升高。食用K8/LC9还增加了内皮型一氧化氮合酶的磷酸化。益生菌治疗引起了SHR盲肠微生物群的变化，乳酸杆菌属菌群数量增加，拟杆菌属和梭菌属数量减少。
益生菌在与血管促氧化和促炎状态改善相关的遗传性高血压中发挥心血管保护作用。
