Research Center for Human Tissues and Organs Degeneration, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, PR China; Shenzhen Key Laboratory of Marine Biomedical Materials, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, PR China.
Research Center for Human Tissues and Organs Degeneration, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, PR China.
Carbohydr Polym. 2015 Nov 5;132:295-303. doi: 10.1016/j.carbpol.2015.06.030. Epub 2015 Jun 18.
Antarctic krill chitosan (A-Chitosan) was first evaluated in its hemostatic effect in this study. The prepared A-Chitosan powder showed low level of crystallinity and significantly high water binding capacity as 1293% (w/w). By mice tail amputation model and blood coagulation timing experiment, it is showed that this chitosan accelerated the tail hemostasis by 55% and shortened the blood clotting time by 38%. This efficacy was better than two other commercial chitosans investigated and was corresponding to their water binding capacities. Through examining the effect of chitosan on blood components, it could be found that platelets adhesion was mainly affected by the water binding capacity, and red blood cells aggregation was dependent on their deacetylation degree. The physicochemical properties resulted in better hydration property of chitosan would improve its hemostatic effect. These results suggested that Antarctic krill chitosan is a good candidate for hemostatic application.
本研究首次评估了南极磷虾壳聚糖（A-Chitosan）的止血效果。制备的 A-Chitosan 粉末显示出低结晶度和显著高的水结合能力为 1293%（w/w）。通过小鼠尾切断模型和血液凝固时间实验，表明该壳聚糖可使尾巴止血时间缩短 55%，凝血时间缩短 38%。这种效果优于两种其他研究的商业壳聚糖，并且与它们的水结合能力相对应。通过检查壳聚糖对血液成分的影响，可以发现血小板黏附主要受水结合能力的影响，而红细胞聚集则依赖于其脱乙酰度。壳聚糖的物理化学性质导致更好的水合性质将提高其止血效果。这些结果表明，南极磷虾壳聚糖是一种很好的止血应用候选材料。
