Key laboratory of Medical Equipment, Academy of Military Medical Sciences,No. 106 Wan Dong Road, He Dong District, Tianjin, 300161, China.
Key laboratory of Medical Equipment, Academy of Military Medical Sciences,No. 106 Wan Dong Road, He Dong District, Tianjin, 300161, China; Department of Textile, Tianjin Polytechnic University,No. 399 Bin Shui West Road, Xi Qing District, Tianjin, 300387, China.
Carbohydr Polym. 2017 Oct 1;173:259-268. doi: 10.1016/j.carbpol.2017.05.085. Epub 2017 Jun 1.
N-Alkylated chitosan (NACS) may improve the haemostatic efficiency of chitosan (CS). To study its coagulation capability and function, a series of NACS with various carbon chain lengths and substitution degrees (SD) of alkyl groups were synthesized and characterized by FTIR, NMR, and elemental analysis. Haemolysis and toxicity assays revealed that NACS showed good biocompatibility. In vitro blood clotting tests indicated that NACS had better haemostatic activity than CS, of which N-octadecyl CS with 3.85% SD showed the best results. Blood plasma coagulation tests showed that NACS was not favourable for activating coagulation factors. Platelet adhesion, intracellular Ca, and CD62p measurements demonstrated that the coagulation properties of NACS were not related to platelet activation. Erythrocyte adhesion examination indicated that blood coagulation of NACS may be attributable to its effects on erythrocytes. This study suggests that NACS is an ideal candidate for clotting.
N- 烷基化壳聚糖（NACS）可能会提高壳聚糖（CS）的止血效率。为了研究其凝血性能和功能，我们合成了一系列具有不同碳链长度和取代度（SD）的 NACS，并通过傅里叶变换红外光谱（FTIR）、核磁共振（NMR）和元素分析对其进行了表征。溶血和毒性试验表明，NACS 具有良好的生物相容性。体外凝血试验表明，NACS 的止血活性优于 CS，其中取代度为 3.85%的 N-十八烷基 CS 表现出最好的效果。血浆凝血试验表明，NACS 不利于激活凝血因子。血小板黏附、细胞内 Ca 和 CD62p 测量表明，NACS 的凝血特性与血小板激活无关。红细胞黏附试验表明，NACS 的血液凝固可能归因于其对红细胞的作用。这项研究表明，NACS 是一种理想的凝血剂候选物。
