Zhang Kexin, Zhao Panpan, Guo Guangyang, Guo Ying, Li Siwen, He Ying, Sun Xiao, Chai Hongliang, Zhang Wen, Xing Mingwei
College of Wildlife Resources, Northeast Forestry University, 26 Hexing Road, Harbin, Heilongjiang Province, 150040, China.
Biol Trace Elem Res. 2016 Apr;170(2):459-65. doi: 10.1007/s12011-015-0487-0. Epub 2015 Sep 2.
Arsenic is a trace element widely found in nature, and there are several forms of arsenic, including the most toxic form of trivalent arsenic. Arsenic trioxide (As2O3) is widespread in nature and tends to accumulate in animals and humans, thus causing great harm. Although the important role of heat shock proteins (HSPs) has been demonstrated in many types of mammals exposed to As2O3, the function of these proteins in poultry, especially in cocks, remains unclear. In this study, we used experimental animals (male chickens), which were fed a diet including 0, 7.5, 15, and 30 mg kg(-1) As2O3, respectively, in the control, low, middle, and high groups. The livers were collected after the cocks were treated with arsenic for 30, 60, and 90 days. We detected HSP27, HSP60, HSP70, and HSP90 levels in the livers of the cocks by real-time PCR and HSP60 and HSP70 levels by Western blot. The results showed that the messenger RNA and protein expression of HSPs exposed to As2O3 had obviously increased. These results demonstrated that arsenic toxicity affected the expression of HSP levels in cock livers.
砷是一种广泛存在于自然界的微量元素，砷有多种形态，包括毒性最强的三价砷。三氧化二砷（As2O3）在自然界广泛存在，并易于在动物和人体内蓄积，从而造成极大危害。尽管热休克蛋白（HSPs）在许多暴露于As2O3的哺乳动物中的重要作用已得到证实，但这些蛋白在家禽尤其是公鸡中的功能仍不清楚。在本研究中，我们使用实验动物（雄性鸡），分别在对照组、低剂量组、中剂量组和高剂量组给予含0、7.5、15和30 mg kg(-1) As2O3的饲料。在公鸡经砷处理30、60和90天后采集肝脏。我们通过实时PCR检测公鸡肝脏中HSP27、HSP60、HSP70和HSP90的水平，并通过蛋白质免疫印迹法检测HSP60和HSP70的水平。结果表明，暴露于As2O3的热休克蛋白的信使核糖核酸和蛋白质表达明显增加。这些结果证明砷毒性影响公鸡肝脏中热休克蛋白水平的表达。
