Cellular Response to Linear and Branched Poly(acrylic acid).

作者信息

Whitty Elizabeth G, Maniego Alison R, Bentwitch Sharon A, Guillaneuf Yohann, Jones Mark R, Gaborieau Marianne, Castignolles Patrice

机构信息

University of Western Sydney, Molecular Medicine Research Group, Locked Bag 1797, Penrith, New South Wales, 2751, Australia.

University of Western Sydney, Australian Centre for Research on Separation Science (ACROSS), Locked Bag 1797, Penrith, New South Wales, 2751, Australia.

出版信息

Macromol Biosci. 2015 Dec;15(12):1724-34. doi: 10.1002/mabi.201500153. Epub 2015 Aug 10.

DOI:10.1002/mabi.201500153

PMID:26257305

Abstract

Poly(acrylic acid-co-sodium acrylate) (PNaA) is a pH-responsive polymer with potential in anticancer drug delivery. The cytotoxicity and intracellular effects of 3-arm star, hyperbranched and linear PNaA were investigated with L1210 progenitor leukemia cells and L6 myoblast cells. Free solution capillary electrophoresis demonstrated interactions of PNaA with serum proteins. In a 72 h MTT assay most PNaAs exhibited a IC50 between 7 and 14 mmol L(-1), showing that precipitation may be a sufficient purification for PNaA dilute solutions. Dialyzed 3-arm star and hyperbranched PNaA caused an increase in L6 cell viability, challenging the suitability of MTT as cytotoxicity assay for PNaA. Fluorescent confocal microscopy revealed merging of cellular lipids after exposure to PNaA, likely caused by serum starvation.

摘要