Rozo-Lopez Paula, Mengual Ximo
Rheinische Friedrich-Wilhelms-Universität Bonn. An der Immenburg 1, 53121 Bonn, Germany.
Zoologisches Forschungsmuseum Alexander Koenig. Adenauerallee 160, 53113 Bonn, Germany.
Zookeys. 2015 Jul 15(513):39-64. doi: 10.3897/zookeys.513.9561. eCollection 2015.
Colombia, one of the world's megadiverse countries, has a highly diverse mosquito fauna and a high prevalence of mosquito-borne diseases. In order to provide relevant information about the diversity and taxonomy of mosquito species in Colombia and to test the usefulness of DNA barcodes, mosquito species collected at different elevations in the departments of Antioquia and Caldas were identified combining adult morphology and barcode sequences. A total of 22 mosquito species from eight genera were identified using these combined techniques. We generated 77 barcode sequences with 16 species submitted as new country records for public databases. We examined the usefulness of DNA barcodes to discriminate mosquito species from the Neotropics by compiling 1,292 sequences from a total of 133 species and using the tree-based methods of neighbor-joining and maximum likelihood. Both methodologies provided similar results by resolving 105 species of mosquitoes separated into distinct clusters. This study shows the importance of combining classic morphological methodologies with molecular tools to accurately identify mosquitoes from Colombia.
哥伦比亚是世界上生物多样性极为丰富的国家之一，拥有种类繁多的蚊子种群，且蚊媒疾病的流行率很高。为了提供有关哥伦比亚蚊子种类多样性和分类学的相关信息，并测试DNA条形码的实用性，我们结合成虫形态学和条形码序列，对在安蒂奥基亚省和卡尔达斯省不同海拔采集的蚊子种类进行了鉴定。使用这些综合技术共鉴定出了来自8个属的22种蚊子。我们生成了77个条形码序列，其中16个物种作为新的国家记录提交到了公共数据库。我们通过汇编来自总共133个物种的1292个序列，并使用基于树的邻接法和最大似然法，研究了DNA条形码区分新热带地区蚊子种类的实用性。两种方法都通过解析分为不同类群的105种蚊子得出了相似的结果。这项研究表明，将经典形态学方法与分子工具相结合对于准确鉴定来自哥伦比亚的蚊子具有重要意义。
