Universidad Cooperativa de Colombia, Centro de Investigación en Salud para el Trópico, Santa Marta, Colombia.
Universidad de La Salle, Departamento de Ciencias Básicas, Programa de Biología, Bogotá, Colombia.
Rev Inst Med Trop Sao Paulo. 2021 Mar 24;63:e24. doi: 10.1590/S1678-9946202163024. eCollection 2021.
A few studies have carried out the taxonomic and molecular characterization of sylvatic mosquito species in Latin America, where some species have been incriminated as vectors for arboviruses and parasites transmission. The present study reports the molecular characterization of mosquito species in the Sierra Nevada de Santa Marta, a natural ecosystem in the Northern coast of Colombia. Manual capture methods were used to collect mosquitoes, and the specimens were identified via classical taxonomy. The COI marker was used for species confirmation, and phylogenetic analysis was performed using the neighbor-joining method, with the Kimura-2-Parameters model. Aedes serratus , Psorophora ferox , Johnbelkinia ulopus , Sabethes chloropterus , Sabethes cyaneus , Wyeomyia aporonoma , Wyeomyia pseudopecten , Wyeomyia ulocoma and Wyeomyia luteoventralis were identified. We assessed the genetic variability of mosquitoes in this area and phylogenetic reconstructions allowed the identification at the species level. Classical and molecular taxonomy demonstrated to be useful and complementary when morphological characteristics are not well preserved, or the taxonomic group is not represented in public molecular databases.
一些研究已经对拉丁美洲的森林蚊种进行了分类学和分子特征描述，其中一些蚊种已被确定为虫媒病毒和寄生虫传播的媒介。本研究报告了哥伦比亚北部沿海自然生态系统内的内华达山脉的蚊种的分子特征描述。使用手动捕获方法收集蚊子，并通过经典分类学方法对标本进行鉴定。使用 COI 标记进行物种确认，并使用邻接法和 Kimura-2-Parameters 模型进行系统发育分析。鉴定出了 Aedes serratus、Psorophora ferox、Johnbelkinia ulopus、Sabethes chloropterus、Sabethes cyaneus、Wyeomyia aporonoma、Wyeomyia pseudopecten、Wyeomyia ulocoma 和 Wyeomyia luteoventralis。我们评估了该地区蚊子的遗传变异性，系统发育重建允许在物种水平上进行鉴定。当形态特征保存不佳或分类群未在公共分子数据库中代表时，经典分类学和分子分类学证明是有用且互补的。
