Chen Hao, Lin Lingling, Chen Xiao, Ai Weiming, Chen Shaobo
a Department of Marine Biotechnology , School of Life Science, Wenzhou Medical University , Wenzhou , Zhejiang , PR China and.
b Zhejiang Mariculture Research Institute , Wenzhou , Zhejiang , PR China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Jul;27(4):3045-7. doi: 10.3109/19401736.2015.1063127. Epub 2015 Aug 10.
In this study, the complete mitochondrial genome of the Blotchy swell shark Cephaloscyllium umbratile was determined. It was a circle molecular (16 698 bp), contained 37 genes with typical order to that of most other vertebrates. The nucleotide composition was 31.0% A, 24.0% C, 14.0% G, and 31.3% T. There were 26 bp short intergenic spaces located in 11 gene junctions and 28 bp overlaps located in 7 gene junctions in the whole mitogenome. Two start codons (GTG and ATG) and two stop codons (TAG and TAA/T) were used in the protein-coding genes. The phylogenetic result showed that C. umbratile was clustered with Scyliorhinus canicula and formed the Scyliorhinidae clade, which was the most basal clade within Carcharhiniformes, and Carcharhinidae is not monophyletic.
在本研究中,测定了斑点皱唇鲨(Cephaloscyllium umbratile)的完整线粒体基因组。它是一个环状分子(16698 bp),包含37个基因,其排列顺序与大多数其他脊椎动物的典型顺序相同。核苷酸组成为31.0%的A、24.0%的C、14.0%的G和31.3%的T。在整个线粒体基因组中,11个基因连接处存在26 bp的短基因间隔,7个基因连接处存在28 bp的重叠。蛋白质编码基因使用了两个起始密码子(GTG和ATG)和两个终止密码子(TAG和TAA/T)。系统发育结果表明,斑点皱唇鲨与小斑猫鲨(Scyliorhinus canicula)聚类,形成了皱唇鲨科分支,这是真鲨目内最基部的分支,并且真鲨科不是单系的。