[中间普雷沃菌表面提取物对小鼠脾淋巴细胞的毒性]

[Toxicity of Bacteroides intermedius surface extracts against mouse splenic lymphocyte].

作者信息

Kuroki T, Kato H, Nishihara Y, Nakagawa S, Makimura M, Otake S

出版信息

Nichidai Koko Kagaku. 1989 Jun;15(2):172-5.

Abstract

The purpose of the present study is to clarify in vitro toxicity of surface sonic extracts from Bacteroides intermedius (B. intermedius) ATCC25611, B. intermedius ATCC 33563 and Bacteroides gingivalis (B. gingivalis) 381 against mouse splenic lymphocyte. Especially the surface extracts from B. intermedius ATCC 25611 were fractionated by high performance liquid chromatography (HPLC) on a column of DEAE. The following results were obtained: 1) The surface extracts from B. gingivalis 381 and B. intermedius ATCC 25611 presented remarkable lymphotoxicity, but B. intermedius ATCC33563 did not have it. 2) The surface extracts from B. intermedius ATCC 25611 was separated to 6 fractions by HPLC. 3) Two fractions isolated from B. intermedius ATCC 25611 surface extracts observed remarkable lymphotoxicity, and the one of their fractions, possibly including fimbriae showed a remarkably higher toxicity against mouse splenic lymphocyte.

摘要

本研究的目的是阐明中间普氏菌（B. intermedius）ATCC25611、中间普氏菌ATCC 33563和牙龈卟啉单胞菌（B. gingivalis）381的表面超声提取物对小鼠脾淋巴细胞的体外毒性。特别是中间普氏菌ATCC 25611的表面提取物通过在DEAE柱上的高效液相色谱（HPLC）进行分级分离。得到以下结果：1）牙龈卟啉单胞菌381和中间普氏菌ATCC 25611的表面提取物表现出显著的淋巴细胞毒性，但中间普氏菌ATCC33563没有。2）中间普氏菌ATCC 25611的表面提取物通过HPLC分离为6个级分。3）从中间普氏菌ATCC 25611表面提取物中分离出的两个级分表现出显著的淋巴细胞毒性，其中一个可能包含菌毛的级分对小鼠脾淋巴细胞表现出显著更高的毒性。