Kayser K, Schmidt A, Stute H, Bach S
Department of Pathology, Thoraxklinik Heidelberg, Rohrbach, FRG.
Pathol Res Pract. 1989 Nov;185(5):584-8. doi: 10.1016/S0344-0338(89)80198-0.
Tumour imprints of 75 resection specimens with human lung carcinoma (lobe and lungs) were fixed with alcohol and Feulgen-stained. Resection specimens were cut into serial sections 6 mm thick and tumour mass, tumour involvement into intrapulmonary and extrapulmonary lymph nodes, detailed pTN-stage were determined. DNA-content, DNA-index, percentage of diploid/hyperploid tumour cells, and morphometric nuclear features were measured using an automated image analyzing system (VISIAC). Only 10% of the measured carcinomas were diploid. The DNA-index showed the broadest variance in large cell anaplastic carcinoma (1.2-3.3). Carcinomas growing predominantly within the alveolar space, i.e. without destroying the interstitial tissue showed a lower DNA-content above 3c and above 5c compared to carcinomas destroying the interstitial tissue. Carcinomas with severe stroma reaction were found to have 33%-48% of DNA above the 3c value whereas carcinomas without stromal reaction had a percentage above 3c ranging 46%-64% (confidence limits, p less than 0.05). DNA-index increases with increasing tumour volume and decreases for large tumours (greater than 100 ccm). Hyperploid and polyploid tumours were found more frequently in case of pT2 and pT3-stages compared to pT1-stages. No relation of DNA-content or ploidy was found to lymph node involvement and inflammatory response of host tissue.
对75例人肺癌（肺叶和全肺）切除标本的肿瘤印片用酒精固定并进行福尔根染色。将切除标本切成6毫米厚的连续切片，确定肿瘤大小、肿瘤累及肺内和肺外淋巴结情况以及详细的pTN分期。使用自动图像分析系统（VISIAC）测量DNA含量、DNA指数、二倍体/超二倍体肿瘤细胞百分比以及形态计量学核特征。所测癌组织中仅10%为二倍体。DNA指数在大细胞间变癌中变化范围最广（1.2 - 3.3）。与破坏间质组织的癌相比，主要在肺泡腔内生长（即不破坏间质组织）的癌在3c以上和5c以上的DNA含量较低。发现有严重间质反应的癌33% - 48%的DNA高于3c值，而无间质反应的癌高于3c的百分比范围为46% - 64%（置信限，p小于0.05）。DNA指数随肿瘤体积增大而升高，大肿瘤（大于100立方厘米）则降低。与pT1期相比，pT2和pT3期更常发现超二倍体和多倍体肿瘤。未发现DNA含量或倍性与淋巴结受累及宿主组织炎症反应之间存在关联。
