Kayser K, Stute H, Tacke M
Department of Pathology, Thoraxklinik, Heidelberg, Germany.
Anal Cell Pathol. 1993 Jul;5(4):225-34.
Surgical specimens comprising 80 primary lung carcinoma (lobes and lungs) were expanded by insufflation of air into the main bronchi, and fixed with buffered formalin for 24 h. After the usual tissue procedures, 3-4 microns thick histological sections of the tumour mass were Feulgen stained and various nuclear features such as integrated optical density (IOD), area, form factor, etc. were measured using an automated image analysing system (VISIAC). The geometrical centres of the nuclei were defined as vertices and the corresponding minimum spanning tree (MST) was calculated according to the distance between the vertices. The tumour mass was measured by serial sections of the surgical specimens; the lymph node stage was defined according to the rules of the UICC. Non-tumour infiltrated lymph nodes of the same case served as controls for the IOD and MST. The results revealed a DNA index of 1.1-3.0, a malignancy index (Böcking) 0.90-1.08 and a percentage of S-phases 10-23% (confidence limits). Only 19% of the bronchial carcinoma were found to be not aneuploid. Based upon the weighted MST, the distance of neighbouring cells, the IOD of the centre cells and IOD/area of neighbouring cells revealed statistically significant differences between tumours with and without lymph node metastases. The more advanced the lymph node stage, the 'closer' was the 'packing' of the tumour cells.
80例原发性肺癌（肺叶和全肺）手术标本通过向主支气管内注入空气进行扩张，并用缓冲甲醛固定24小时。经过常规组织处理后，对肿瘤块制作3 - 4微米厚的组织学切片，进行福尔根染色，并使用自动图像分析系统（VISIAC）测量各种核特征，如积分光密度（IOD）、面积、形态因子等。将细胞核的几何中心定义为顶点，并根据顶点之间的距离计算相应的最小生成树（MST）。通过手术标本的连续切片测量肿瘤块；根据国际抗癌联盟（UICC）的规则定义淋巴结分期。同一病例的非肿瘤浸润淋巴结用作IOD和MST的对照。结果显示DNA指数为1.1 - 3.0，恶性指数（博金指数）为0.90 - 1.08，S期百分比为10 - 23%（置信限）。仅19%的支气管癌被发现为非非整倍体。基于加权MST，相邻细胞的距离、中心细胞的IOD以及相邻细胞的IOD/面积显示有淋巴结转移和无淋巴结转移的肿瘤之间存在统计学上的显著差异。淋巴结分期越晚，肿瘤细胞的“堆积”越“紧密”。
