Kayser K, Liewald F, Kremer K, Tacke M
Department of Pathology, Thoraxklinik, Heidelberg, FRG.
Pathol Res Pract. 1994 Nov;190(11):1031-8. doi: 10.1016/S0344-0338(11)80898-8.
Histological sections of formalin fixed, paraffin-embedded tissue comprising 195 specimens of human lung carcinomas were Feulgen stained. The nuclei of the histomorphological images were segmented using an automated image analyzing system, and the attributed minimum spanning trees (MST) were calculated. Features related to the DNA-content of the nuclei (integrated optical density (IOD), IOD-entropy, S-phase related fraction, percentage > 5C, etc.), and structural parameters (minimum distance between tumor cell nuclei, minimum distance between tumor cells and neighboring lymphocytes, MST-entropy, MST-current of entropy (entropiefluss), distance between neighboring proliferating tumor cells, etc.) were measured. The following results were obtained: the measured IOD and MST features showed significant differences between the primary carcinomas and metastatic carcinomas in the intrapulmonary lymph nodes. The survival of patients was remarkably improved if the carcinomas displayed a low S-phase related fraction, a low percentage of tumor cells > 5C, a low number of stem lines, and a low MST-current of entropy.
对195例人类肺癌的福尔马林固定、石蜡包埋组织的组织学切片进行Feulgen染色。使用自动图像分析系统对组织形态学图像的细胞核进行分割，并计算归属的最小生成树（MST）。测量与细胞核DNA含量相关的特征（积分光密度（IOD）、IOD熵、S期相关分数、>5C的百分比等）以及结构参数（肿瘤细胞核之间的最小距离、肿瘤细胞与相邻淋巴细胞之间的最小距离、MST熵、MST熵流、相邻增殖肿瘤细胞之间的距离等）。获得以下结果：所测量的IOD和MST特征在原发性癌和肺内淋巴结转移癌之间显示出显著差异。如果癌显示出低S期相关分数、低百分比的肿瘤细胞>5C、低干细胞系数量和低MST熵流，则患者的生存率显著提高。
