Jung Yu Jung, Kwon Hyeon Jeong, Huh Hee Jae, Ki Chang-Seok, Lee Nam Yong, Kim Jong-Won
Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea.
Center for Clinical Medicine, Samsung Biomedical Research Institute, Samsung Medical Center, Seoul, Republic of Korea.
J Virol Methods. 2015 Nov;224:42-6. doi: 10.1016/j.jviromet.2015.08.003. Epub 2015 Aug 13.
The AdvanSure™ RV real-time PCR kit (AdvanSure; LG Life Sciences, Korea) is based on multiplex real-time PCR and can simultaneously detect 14 respiratory viruses. We compared the performance of the AdvanSure assay with the Seeplex RV 12 ACE detection kit (Seeplex; Seegene, Seoul, South Korea), a multiplex end-point PCR assay. A total of 454 consecutive respiratory specimens were tested with both AdvanSure and Seeplex assays; AdvanSure detected 153 (33.7%) positive cases and Seeplex detected 145 (31.9%) positive cases. The positive percent agreement, negative percent agreement, and kappa value for the two assays were 87.2% (95% CI, 80.3-92.1), 91.1% (95% CI, 87.2-93.9), and 0.77 (95% CI, 0.70-0.83), respectively. Compared with the Seeplex assay, the AdvanSure assay had a shorter turnaround time (3h vs. 8h) and a shorter hands-on time (<1h vs 2h). In conclusion, the AdvanSure assay demonstrated comparable performance to the Seeplex assay.
AdvanSure™ RV实时荧光定量PCR检测试剂盒（AdvanSure；韩国LG生命科学公司）基于多重实时荧光定量PCR技术，可同时检测14种呼吸道病毒。我们将AdvanSure检测方法的性能与多重终点PCR检测方法Seeplex RV 12 ACE检测试剂盒（Seeplex；韩国首尔Seegene公司）进行了比较。共有454份连续的呼吸道标本同时采用AdvanSure和Seeplex检测方法进行检测；AdvanSure检测出153例（33.7%）阳性病例，Seeplex检测出145例（31.9%）阳性病例。两种检测方法的阳性一致率、阴性一致率和kappa值分别为87.2%（95%CI，80.3 - 92.1）、91.1%（95%CI，87.2 - 93.9）和0.77（95%CI，0.70 - 0.83）。与Seeplex检测方法相比，AdvanSure检测方法的周转时间更短（3小时对8小时），实际操作时间更短（<1小时对2小时）。总之，AdvanSure检测方法与Seeplex检测方法表现出相当的性能。
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