Department of Environmental Sciences, Rutgers University , 14 College Farm Road, New Brunswick, New Jersey 08901, United States.
DuPont, Corporate Remediation Group, Wilmington, Delaware 19714, United States.
Environ Sci Technol. 2015 Sep 15;49(18):11079-88. doi: 10.1021/acs.est.5b02166. Epub 2015 Aug 26.
Anaerobic aniline biodegradation was investigated under different electron-accepting conditions using contaminated canal and groundwater aquifer sediments from an industrial site. Aniline loss was observed in nitrate- and sulfate-amended microcosms and in microcosms established to promote methanogenic conditions. Lag times of 37 days (sulfate amended) to more than 100 days (methanogenic) were observed prior to activity. Time-series DNA-stable isotope probing (SIP) was used to identify bacteria that incorporated (13)C-labeled aniline in the microcosms established to promote methanogenic conditions. In microcosms from heavily contaminated aquifer sediments, a phylotype with 92.7% sequence similarity to Ignavibacterium album was identified as a dominant aniline degrader as indicated by incorporation of (13)C-aniline into its DNA. In microcosms from contaminated canal sediments, a bacterial phylotype within the family Anaerolineaceae, but without a match to any known genus, demonstrated the assimilation of (13)C-aniline. Acidovorax spp. were also identified as putative aniline degraders in both of these two treatments, indicating that these species were present and active in both the canal and aquifer sediments. There were multiple bacterial phylotypes associated with anaerobic degradation of aniline at this complex industrial site, which suggests that anaerobic transformation of aniline is an important process at the site. Furthermore, the aniline degrading phylotypes identified in the current study are not related to any known aniline-degrading bacteria. The identification of novel putative aniline degraders expands current knowledge regarding the potential fate of aniline under anaerobic conditions.
采用受污染的运河和地下水含水层沉积物，在不同电子受体条件下研究了厌氧苯胺生物降解。在添加硝酸盐和硫酸盐的微宇宙中和在促进产甲烷条件的微宇宙中观察到苯胺的损失。在活动之前，硫酸盐添加的滞后时间为 37 天（硫酸盐添加）至 100 天以上（产甲烷）。时间序列 DNA 稳定同位素探针（SIP）用于鉴定在促进产甲烷条件的微宇宙中掺入（13）C 标记苯胺的细菌。在受污染含水层沉积物的微宇宙中，与 Ignavibacterium album 相似度为 92.7%的一个生物型被鉴定为主要的苯胺降解菌，因为它的 DNA 中掺入了（13）C-苯胺。在受污染运河沉积物的微宇宙中，属于 Anaerolineaceae 科的细菌生物型，但与任何已知属都不匹配，证明其同化了（13）C-苯胺。在这两种处理中，还鉴定出 Acidovorax spp. 是潜在的苯胺降解菌，表明这些物种存在于运河和含水层沉积物中并且活跃。在这个复杂的工业场地，有多个与苯胺厌氧降解相关的细菌生物型，这表明苯胺的厌氧转化是该场地的一个重要过程。此外，本研究中鉴定的苯胺降解生物型与任何已知的苯胺降解细菌都没有关系。新型潜在苯胺降解菌的鉴定扩展了关于在厌氧条件下苯胺潜在命运的现有知识。
