Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1GA, UK.
Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge CB2 1PD, UK.
Curr Opin Struct Biol. 2015 Aug;33:76-91. doi: 10.1016/j.sbi.2015.07.015. Epub 2015 Aug 15.
Cells from all domains of life encode energy-dependent trans-membrane transporters that can expel harmful substances including clinically applied therapeutic agents. As a collective body, these transporters perform as a super-system that confers tolerance to an enormous range of harmful compounds and consequently aid survival in hazardous environments. In the Gram-negative bacteria, some of these transporters serve as energy-transducing components of tripartite assemblies that actively efflux drugs and other harmful compounds, as well as deliver virulence agents across the entire cell envelope. We draw together recent structural and functional data to present the current models for the transport mechanisms for the main classes of multi-drug transporters and their higher-order assemblies.
所有生命领域的细胞都编码能量依赖性的跨膜转运蛋白，这些转运蛋白可以排出有害物质，包括临床应用的治疗剂。作为一个整体，这些转运蛋白表现为一个超级系统，赋予对大量有害物质的耐受性，从而有助于在危险环境中生存。在革兰氏阴性细菌中，这些转运蛋白中的一些作为三联体组装的能量转换成分，主动排出药物和其他有害物质，并将毒力因子输送穿过整个细胞包膜。我们汇集了最近的结构和功能数据，提出了主要多药物转运蛋白及其高级组装体的运输机制的现行模型。
