Abd El-Fatah Marwa F, Ghazy Mohamed A, Mostafa Mohamed S, El-Attar May M, Osman Ahmed
a Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo 11566, Egypt.
b Poisoning Control Center, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.
Biochem Cell Biol. 2015 Dec;93(6):541-7. doi: 10.1139/bcb-2015-0073. Epub 2015 Jul 20.
Chronic obstructive pulmonary disease (COPD) is a complex immunological disease with multiple pathological features that is primarily induced by smoking together with additional genetic risk factors. COPD is frequently underdiagnosed; forced expiratory volume in the first second (FEV1) is considered to be the main diagnostic measure for COPD, yet it is insufficiently sensitive to monitor disease progression. Biomarkers capable of monitoring COPD progression and severity are needed. In this report, we evaluated matrix metalloproteinase-9 (MMP-9) as an early marker for the detection and staging of COPD, by assessing the mRNA levels of MMP-9 in peripheral blood samples collected from 22 COPD patients, 6 asymptomatic smokers, and 5 healthy controls. Our results demonstrate that the mRNA levels of MMP-9 increased more than two-fold in severe COPD relative to non-COPD smokers or moderate COPD groups. Moreover, in the very severe COPD group, MMP-9 mRNA levels showed a 4-fold increase relative to the non-COPD smokers or the moderate COPD groups, while there was a mild increase (∼40%) when compared to the severe COPD group. Taken together, our results suggest that MMP-9 serves as a biomarker for the grade and severity of COPD.
慢性阻塞性肺疾病（COPD）是一种具有多种病理特征的复杂免疫性疾病，主要由吸烟以及其他遗传风险因素诱发。COPD常常诊断不足；第一秒用力呼气容积（FEV1）被认为是COPD的主要诊断指标，但它对监测疾病进展的敏感性不足。因此需要能够监测COPD进展和严重程度的生物标志物。在本报告中，我们通过评估从22例COPD患者、6例无症状吸烟者和5例健康对照者采集的外周血样本中基质金属蛋白酶-9（MMP-9）的mRNA水平，来评估MMP-9作为COPD检测和分期的早期标志物。我们的结果表明，与非COPD吸烟者或中度COPD组相比，重度COPD患者中MMP-9的mRNA水平增加了两倍多。此外，在极重度COPD组中，与非COPD吸烟者或中度COPD组相比，MMP-9 mRNA水平增加了4倍，而与重度COPD组相比则有轻度增加（约40%）。综上所述，我们的结果表明MMP-9可作为COPD分级和严重程度的生物标志物。
