Das Diganta, Sharma Maitreyee, Kumar Das Hemanga, Pratim Sahu Partha, Doley Robin
Molecular Toxinology Laboratory, Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur-, 784028, India.
Department of Electronics and Communication Engineering, Tezpur University, Tezpur, 784028, India.
J Biochem Mol Toxicol. 2016 Feb;30(2):59-70. doi: 10.1002/jbt.21734. Epub 2015 Aug 21.
Snake venom three finger toxins (3FTxs) are a non-enzymatic family of venom proteins abundantly found in elapids. We have purified a 7579.5 ± 0.591 Da 3FTx named as Nk-3FTx from the venom of Naja kaouthia of North East India origin. The primary structure was determined by a combination of N-terminal sequencing and electrospray ionization liquid chromatography-mass spectrometry/mass spectrometry. Biochemical and biological characterization reveal that it is nontoxic to human cell lines and exhibit mild anticoagulant activity when tested on citrated human plasma. Nk-3FTx was found to affect the compound action potential (CAP) and nerve conduction velocity of isolated toad sciatic nerve. This is the first report of a non-conventional 3FTx from Naja kaouthia venom that reduces CAP for its neurotoxic effect. Further studies can be carried out to understand the mechanism of action and to explore its potential therapeutic application.
蛇毒三指毒素（3FTxs）是一类非酶类毒液蛋白家族，在眼镜蛇科中大量存在。我们从原产于印度东北部的眼镜蛇毒液中纯化出一种名为Nk - 3FTx的7579.5 ± 0.591 Da的3FTx。其一级结构通过N端测序和电喷雾电离液相色谱 - 质谱/质谱联用技术确定。生化和生物学特性表明，它对人类细胞系无毒，并且在枸橼酸化人血浆中测试时表现出轻度抗凝活性。发现Nk - 3FTx会影响离体蟾蜍坐骨神经的复合动作电位（CAP）和神经传导速度。这是关于眼镜蛇毒液中一种非常规3FTx的首次报道，该毒素因其神经毒性作用而降低CAP。可以进一步开展研究以了解其作用机制并探索其潜在的治疗应用。
