Scheible Max B, Ong Luvena L, Woehrstein Johannes B, Jungmann Ralf, Yin Peng, Simmel Friedrich C
Physics Department and ZNN/WSI, Technische Universität München, Am Coulombwall 4a, 85748, Garching, Germany.
Nanosystems Initiative Munich, Schellingstr. 4, 80799, München, Germany.
Small. 2015 Oct 21;11(39):5200-5. doi: 10.1002/smll.201501370. Epub 2015 Aug 21.
A small and compact DNA cube with zeptoliter volume is constructed by means of a generalized DNA brick concept using short synthetic oligonucleotides with varying lengths. By mimicking design principles from the DNA origami technique, the DNA cube offers higher stability and assembly yields compared to other approaches. Its potential application as nanoscale fluorescent probe is demonstrated using super-resolution imaging.
通过使用不同长度的短合成寡核苷酸,借助广义DNA砖概念构建了一种体积为zeptoliter的小型紧凑DNA立方体。通过模仿DNA折纸技术的设计原理,与其他方法相比,该DNA立方体具有更高的稳定性和组装产率。利用超分辨率成像展示了其作为纳米级荧光探针的潜在应用。