胸腺上皮细胞及其亚群的流式细胞术分析

Flow Cytometry Analysis of Thymic Epithelial Cells and Their Subpopulations.

作者信息

Ohigashi Izumi, Takahama Yousuke

机构信息

Division of Experimental Immunology, Institute for Genome Research, University of Tokushima, 3-18-15 Kuramoto, Tokushima, 770-8503, Japan.

出版信息

Methods Mol Biol. 2016;1323:65-73. doi: 10.1007/978-1-4939-2809-5_5.

DOI:10.1007/978-1-4939-2809-5_5

PMID:26294398

Abstract

The parenchyma of the thymus is compartmentalized into the cortex and the medulla, which are constructed by cortical thymic epithelial cells (cortical TECs, cTECs) and medullary thymic epithelial cells (mTECs), respectively. cTECs and mTECs essentially and differentially regulate the development and repertoire selection of T cells. Consequently, the biology of T cell development and selection includes the study of TECs in addition to the study of developing T cells and other hematopoietic cells including dendritic cells. In this chapter, we describe the methods for flow cytometric analysis and sorting of TECs and their subpopulations, including cTECs and mTECs.

摘要

胸腺实质被分隔为皮质和髓质，它们分别由皮质胸腺上皮细胞（cTECs）和髓质胸腺上皮细胞（mTECs）构成。cTECs和mTECs对T细胞的发育和库选择起着重要的、不同的调节作用。因此，T细胞发育和选择的生物学不仅包括对发育中的T细胞及包括树突状细胞在内的其他造血细胞的研究，还包括对TECs的研究。在本章中，我们描述了用于TECs及其亚群（包括cTECs和mTECs）的流式细胞术分析和分选方法。