大规模分离小鼠胸腺上皮细胞。
Large-Scale Isolation of Mouse Thymic Epithelial Cells.
机构信息
Division of Experimental Immunology, Institute of Advanced Medical Sciences, University of Tokushima, Tokushima, Japan.
Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
出版信息
Methods Mol Biol. 2023;2580:189-197. doi: 10.1007/978-1-0716-2740-2_11.
The thymus is compartmentalized into the cortex and the medulla. Cortical and medullary thymic epithelial cells (TECs) characterize T cell-producing and T cell-selecting functions of cortical and medullary microenvironments in the thymus. Enzymatic digestion of the thymus and flow cytometric isolation of TECs and their subpopulations are useful for molecular and cellular characterization of TECs. However, the cellularity of cTECs and mTECs isolated from mouse thymus is limited. In this chapter, we describe the method for isolation of a large number of TECs using enlarged mouse thymus, which enables biochemical and proteomic analysis of TEC subpopulations.
胸腺分为皮质和髓质。皮质和髓质胸腺上皮细胞（TEC）的特征在于皮质和髓质微环境中产生 T 细胞和选择 T 细胞的功能。胸腺的酶消化和 TEC 及其亚群的流式细胞术分离对于 TEC 的分子和细胞特征分析是有用的。然而，从小鼠胸腺中分离的 cTEC 和 mTEC 的细胞数量有限。在本章中，我们描述了使用放大的小鼠胸腺分离大量 TEC 的方法，该方法可用于 TEC 亚群的生化和蛋白质组学分析。
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