用于快速超灵敏检测前列腺特异性抗原的电纳米孔诊断传感器。
Electrical nanowell diagnostics sensors for rapid and ultrasensitive detection of prostate-specific antigen.
作者信息
Panneer Selvam Anjan, Prasad Shalini, Barrett Thomas W, Kazmierczak Steven C
机构信息
Department of Bioengineering, University of Texas at Dallas, Richardson, TX 75080, USA.
Department of Veterans Affairs Portland, Oregon Health Sciences University, OR, USA.
出版信息
Nanomedicine (Lond). 2015;10(16):2527-36. doi: 10.2217/NNM.15.100. Epub 2015 Aug 21.
AIM
Alumina nanowell based disposable diagnostic biosensor for detecting and quantifying levels of prostate-specific antigen (PSA) from human serum has been designed, fabricated and tested.
MATERIALS & METHODS: The biosensors were designed by integrating nanoporous alumina membranes onto printed circuit board platforms, resulting in the generation of high-density nanowell arrays with gold base electrodes. The size and density of the nanowells were leveraged toward achieving sieving action for size-based exclusion of nonspecific molecules and size-based confinement of the target PSA molecules.
RESULTS & CONCLUSION: We demonstrated PSA detection between 0.01 and 1000 ng/ml and detection and quantification of PSA from a 17 patient cohort validated using the Beckman Access system with >95% correlation.
目的
设计、制造并测试了一种基于氧化铝纳米孔的一次性诊断生物传感器，用于检测和定量人血清中前列腺特异性抗原（PSA）的水平。
材料与方法
通过将纳米多孔氧化铝膜集成到印刷电路板平台上设计生物传感器，从而产生带有金基电极的高密度纳米孔阵列。利用纳米孔的尺寸和密度实现基于尺寸的筛分作用，以排除非特异性分子，并对目标PSA分子进行基于尺寸的限制。
结果与结论
我们证明了该生物传感器能够检测0.01至1000 ng/ml之间的PSA，并且对来自17名患者队列的PSA进行检测和定量，与使用贝克曼Access系统验证的结果具有>95%的相关性。
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